Literature DB >> 7026679

Quantitation of a whole blood assay for human natural killer cell activity.

P C Ottenhof, A Morales, M G Baines.   

Abstract

A method is described for the measurement of human natural killer cell activity using heparinized whole blood in a 51chromium release assay. Fractionation-reconstitution experiments showed that the cytotoxic activity was abolished by removal of the Fc receptor bearing lymphocytes, but not by the elimination of monocytes and granulocytes. Autologous or pooled plasma was not found to possess inherent cytolytic activity. By analogy to an enzyme kinetic reaction, the results were expressed as kinetic lytic units (KLU) which were defined as the maximum number of target cells that could be lysed per unit time per milliliter of whole blood. The buffy coat cytotoxicity (BCC) assay is quick, easy to perform, and suitable for screening and monitoring of natural cytotoxicity. Since this methodology preserves the actual concentration of natural killer cells, it may represent a truer reflection of in vivo events.

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Year:  1981        PMID： 7026679     DOI： 10.1016/0022-1759(81)90159-9

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  9 in total

1.  Continuous stress disrupts immunostimulatory effects of IL-12.

Authors:  Ben Levi; Marganit Benish; Yael Goldfarb; Liat Sorski; Rivka Melamed; Ella Rosenne; Shamgar Ben-Eliyahu
Journal:  Brain Behav Immun       Date:  2011-01-28       Impact factor: 7.217

2.  Relations of plasma ACTH and cortisol levels with the distribution and function of peripheral blood cells in response to a behavioral challenge in breast cancer: an empirical exploration by means of statistical modeling.

Authors:  G van der Pompe; M H Antoni; H J Duievenvoorden; C J Heijnen
Journal:  Int J Behav Med       Date:  1997

3.  The misleading nature of in vitro and ex vivo findings in studying the impact of stress hormones on NK cell cytotoxicity.

Authors:  Neta Gotlieb; Ella Rosenne; Pini Matzner; Lee Shaashua; Liat Sorski; Shamgar Ben-Eliyahu
Journal:  Brain Behav Immun       Date:  2014-12-26       Impact factor: 7.217

4.  PGE2 suppresses NK activity in vivo directly and through adrenal hormones: effects that cannot be reflected by ex vivo assessment of NK cytotoxicity.

Authors:  G Meron; Y Tishler; L Shaashua; E Rosenne; B Levi; R Melamed; N Gotlieb; P Matzner; L Sorski; S Ben-Eliyahu
Journal:  Brain Behav Immun       Date:  2012-11-12       Impact factor: 7.217

5.  Stress impairs the efficacy of immune stimulation by CpG-C: Potential neuroendocrine mediating mechanisms and significance to tumor metastasis and the perioperative period.

Authors:  B Levi; P Matzner; Y Goldfarb; L Sorski; L Shaashua; R Melamed; E Rosenne; G G Page; S Ben-Eliyahu
Journal:  Brain Behav Immun       Date:  2016-03-02       Impact factor: 7.217

6.  Suppression of peripheral blood natural killer cell activity by excess thyroid hormone.

Authors:  M Papic; J Stein-Streilein; M Zakarija; J M McKenzie; J Guffee; M A Fletcher
Journal:  J Clin Invest       Date:  1987-02       Impact factor: 14.808

7.  CpG-C oligodeoxynucleotides limit the deleterious effects of beta-adrenoceptor stimulation on NK cytotoxicity and metastatic dissemination.

Authors:  Yael Goldfarb; Marganit Benish; Ella Rosenne; Rivka Melamed; Ben Levi; Ariella Glasner; Shamgar Ben-Eliyahu
Journal:  J Immunother       Date:  2009-04       Impact factor: 4.456

8.  The marginating-pulmonary immune compartment in mice exhibits increased NK cytotoxicity and unique cellular characteristics.

Authors:  Marganit Benish; Rivka Melamed; Ella Rosenne; Elad Neeman; Liat Sorski; Ben Levi; Lee Shaashua; Pini Matzner; Shamgar Ben-Eliyahu
Journal:  Immunol Res       Date:  2014-01       Impact factor: 2.829

9.  Timing within the menstrual cycle, sex, and the use of oral contraceptives determine adrenergic suppression of NK cell activity.

Authors:  K Shakhar; G Shakhar; E Rosenne; S Ben-Eliyahu
Journal:  Br J Cancer       Date:  2000-12       Impact factor: 7.640
  9 in total

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