Hydrogenase genes.

For a variety of reasons, including the potential industrial applications of hydrogenase, we are interested in the isolation and analysis of hydrogenase genes. In a program focusing on the hydrogen bacterium A. eutrophus H1 and E. coli, we have developed a preliminary concept of the interaction of hydrogenase in cellular metabolism, constructed mutants deficient in hydrogenase activity, and begun the isolation of hydrogenase genes utilizing the technology allowing the in vitro manipulation of DNA. We hope to pursue this project to its ultimate goal: the analysis of the molecular mechanisms involved in the control of expression of these genes and the development of the ability to manipulate the production of hydrogenase.