| Literature DB >> 7021574 |
Abstract
Horseradish peroxidase has been conjugated with human immunoglobulin containing antibody to hepatitis B e antigen (anti-HBe). This was used in a solid-phase 'sandwich' enzyme-linked immunosorbent assay designed for reading by eye, to test sera for HBeAg and anti-HBe. Optimum conditions for preparing the conjugate and for performing the test are described. Results of testing hepatitis B surface antigen (HBsAg)-positive sera were compared with those obtained using a sensitive solid-phase radioimmunoassay. The enzyme assay provided a simple and sensitive method of testing for HBeAg and anti-HBe, and correlated well with the radioimmunoassay.Entities:
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Year: 1981 PMID： 7021574 DOI： 10.1016/0166-0934(81)90017-3
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014