Literature DB >> 7020949

Immunohistochemical localization of cholinergic nerve terminals.

R T Jones, J H Walker, P J Richardson, G Q Fox, V P Whittaker.   

Abstract

Most of the published light-microscopic methods for the localization of cholinergic nerve pathways present various difficulties of interpretation. The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small synaptosomes prepared from the purely cholinergic electric organ of Torpedo marmorata. It was shown to lyse cholinergic synaptosomes in a mixed population derived from guinea-pig cortex. After partial purification by adsorption onto nonspecific antigens, It was used to label nerve endings in several tissues of Torpedo, rat and guinea pigs using indirect immunofluorescence histochemistry. The antiserum appears to provide a highly specific means of localizing cholinergic nerve endings in these tissues.

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Year:  1981        PMID： 7020949     DOI： 10.1007/bf00210350

Source DB:  PubMed          Journal:  Cell Tissue Res        ISSN： 0302-766X            Impact factor:   5.249


  32 in total

1.  A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES.

Authors:  M J KARNOVSKY; L ROOTS
Journal:  J Histochem Cytochem       Date:  1964-03       Impact factor: 2.479

2.  The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation.

Authors:  E G GRAY; V P WHITTAKER
Journal:  J Anat       Date:  1962-01       Impact factor: 2.610

3.  Acetylcholinesterase-staining synaptosomes from rat hippocampus: relative frequency and tentative estimation of internal concentration of free or 'labile bound' acetylcholine.

Authors:  M J Kuhar; H Rommelspacher
Journal:  Brain Res       Date:  1974-08-30       Impact factor: 3.252

4.  Ultrastructural antibody localization of alpha2-macroglobulin in membrane-limited vesicles in cultured cells.

Authors:  M C Willingham; S S Yamada; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

5.  Antibody to bovine choline acetyltransferase and immunofluorescent localisation of the enzyme in neurones.

Authors:  L F Eng; C T Uyeda; L P Chao; F Wolfgram
Journal:  Nature       Date:  1974-07-19       Impact factor: 49.962

6.  The electromotor system of Torpedo. A model cholinergic system.

Authors:  V P Whittaker
Journal:  Naturwissenschaften       Date:  1977-12

7.  Confirmation from choline acetylase analyses of a massive cholinergic innervation to the rat hippocampus.

Authors:  P R Lewis; C C Shute; A Silver
Journal:  J Physiol       Date:  1967-07       Impact factor: 5.182

8.  A radiochemical method for the estimation of choline acetyltransferase.

Authors:  F Fonnum
Journal:  Biochem J       Date:  1966-08       Impact factor: 3.857

9.  Studies of the uptake of 3 H-gaba and ( 3 H)glycine in slices and homogenates of rat brain and spinal cord by electron microscopic autoradiography.

Authors:  L L Iversen; F E Bloom
Journal:  Brain Res       Date:  1972-06-08       Impact factor: 3.252

10.  Antibodies to synaptic vesicles purified from Narcine electric organ bind a subclass of mammalian nerve terminals.

Authors:  J E Hooper; S S Carlson; R B Kelly
Journal:  J Cell Biol       Date:  1980-10       Impact factor: 10.539
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  14 in total

1.  Complement action on secretory cells identified by the reverse hemolytic plaque assay: modified assay eliminates exposure of secretory cells to complement.

Authors:  K A Gregerson
Journal:  Endocrine       Date:  1995-05       Impact factor: 3.633

2.  Cholinergic function in the nineties: advantages of work with a model system.

Authors:  V P Whittaker
Journal:  Neurochem Res       Date:  1987-02       Impact factor: 3.996

3.  Immunoaffinity purification of intact, metabolically active, cholinergic nerve terminals from mammalian brain.

Authors:  P J Richardson; K Siddle; J P Luzio
Journal:  Biochem J       Date:  1984-04-15       Impact factor: 3.857

4.  Immunohistochemical localization of a synaptic-vesicle antigen in a cholinergic neuron under conditions of stimulation and rest.

Authors:  R T Jones; J H Walker; H Stadler; V P Whittaker
Journal:  Cell Tissue Res       Date:  1982       Impact factor: 5.249

5.  Further evidence that glycosaminoglycan specific to cholinergic synaptic vesicles recycles during electrical stimulation of the electric organ of Torpedo marmorata.

Authors:  R T Jones; J H Walker; H Stadler; V P Whittaker
Journal:  Cell Tissue Res       Date:  1982       Impact factor: 5.249

6.  Identification and immunocytochemical localization of a human adult brain-specific antigen (HABSA).

Authors:  I R Kehayov; S D Kyurkchiev; M S Davidoff; P G Galabov
Journal:  Histochemistry       Date:  1983

7.  Immunocytochemical evaluation of a cholinergic-specific ganglioside antigen (Chol-1) in the central nervous system of the rat.

Authors:  J Obrocki; E Borroni
Journal:  Exp Brain Res       Date:  1988       Impact factor: 1.972

Review 8.  Cholinergic-specific glycoconjugates.

Authors:  V P Whittaker; S Kelić
Journal:  Neurochem Res       Date:  1995-11       Impact factor: 3.996

9.  Synaptic function of cholinergic-specific Chol-1alpha ganglioside.

Authors:  Susumu Ando; Yasukazu Tanaka; Satoru Kobayashi; Fumiko Fukui; Machiko Iwamoto; Hatsue Waki; Tadashi Tai; Yoshio Hirabayashi
Journal:  Neurochem Res       Date:  2004-04       Impact factor: 3.996

10.  Presynaptic plasma membranes and synaptic vesicles of cholinergic nerve endings demonstrated by means of specific antisera.

Authors:  J H Walker; R T Jones; J Obrocki; G P Richardson; H Stadler
Journal:  Cell Tissue Res       Date:  1982       Impact factor: 5.249
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