Literature DB >> 7020758

Purification of yeast tubulin by self-assembly in vitro.

J V Kilmartin.   

Abstract

Tubulin was purified from yeast homogenate by DEAE-Sephadex column chromatography and temperature-dependent assembly. The yeast tubulin subunits comigrate with the brain alpha-tubulin subunit on one-dimensional sodium dodecyl sulfate gel electrophoresis. The in vitro yeast tubulin assembly is inhibited by the fungicide methyl N-(benzimidazol-2-yl)carbamate, the active component of benomyl, whereas in vitro brain 6S tubulin assembly is resistant. This suggests that the inhibitory effect of benomyl on yeast cell division is due to its antimicrotubule action.

Entities:  

Mesh：

Substances：

Year:  1981        PMID： 7020758     DOI： 10.1021/bi00515a050

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  64 in total

1.  Dominant effects of tubulin overexpression in Saccharomyces cerevisiae.

Authors:  D Burke; P Gasdaska; L Hartwell
Journal:  Mol Cell Biol       Date:  1989-03       Impact factor: 4.272

2.  A codon change in beta-tubulin which drastically affects microtubule structure in Drosophila melanogaster fails to produce a significant phenotype in Saccharomyces cerevisiae.

Authors:  V Praitis; W S Katz; F Solomon
Journal:  Mol Cell Biol       Date:  1991-09       Impact factor: 4.272

3.  Coordination of Cell Cycle Progression and Mitotic Spindle Assembly Involves Histone H3 Lysine 4 Methylation by Set1/COMPASS.

Authors:  Traude H Beilharz; Paul F Harrison; Douglas Maya Miles; Michael Ming See; Uyen Minh Merry Le; Ming Kalanon; Melissa Jane Curtis; Qambar Hasan; Julie Saksouk; Thanasis Margaritis; Frank Holstege; Vincent Geli; Bernhard Dichtl
Journal:  Genetics       Date:  2016-11-14       Impact factor: 4.562

Review 4.  Antifungal agents: chemotherapeutic targets and immunologic strategies.

Authors:  N H Georgopapadakou; T J Walsh
Journal:  Antimicrob Agents Chemother       Date:  1996-02       Impact factor: 5.191

5.  Isolation of a beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance.

Authors:  K Yan; M B Dickman
Journal:  Appl Environ Microbiol       Date:  1996-08       Impact factor: 4.792

6.  Fission yeast mutants that alleviate transcriptional silencing in centromeric flanking repeats and disrupt chromosome segregation.

Authors:  K Ekwall; G Cranston; R C Allshire
Journal:  Genetics       Date:  1999-11       Impact factor: 4.562

7.  Systematic mutational analysis of the yeast beta-tubulin gene.

Authors:  R A Reijo; E M Cooper; G J Beagle; T C Huffaker
Journal:  Mol Biol Cell       Date:  1994-01       Impact factor: 4.138

8.  Differential expressions of essential and nonessential alpha-tubulin genes in Schizosaccharomyces pombe.

Authors:  Y Adachi; T Toda; O Niwa; M Yanagida
Journal:  Mol Cell Biol       Date:  1986-06       Impact factor: 4.272

9.  The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes.

Authors:  N Sethi; M C Monteagudo; D Koshland; E Hogan; D J Burke
Journal:  Mol Cell Biol       Date:  1991-11       Impact factor: 4.272

10.  Tubulin heterogeneity in the trypanosome Crithidia fasciculata.

Authors:  D G Russell; D Miller; K Gull
Journal:  Mol Cell Biol       Date:  1984-04       Impact factor: 4.272
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.