Literature DB >> 701811

A simple, sensitive assay for determining DNA in mononuclear phagocytes and other leukocytes.

S L Cookson, D O Adams.   

Abstract

An assay for determining the DNA content of mononuclear phagocytes is described. The assay is efficient and very sensitive, measuring as little as 1 microgram of DNA. Content of DNA is a linear function of the number of mononuclear phagocytes in the sample. One million murine macrophages contain 10.1 +/- 0.36 microgram of DNA. Consequently, samples of as few as 100,000 macrophages can be accurately quantified to be related to other biochemical analyses.

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Year:  1978        PMID: 701811     DOI: 10.1016/0022-1759(78)90120-5

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  6 in total

1.  Intralipid alters macrophage membrane fatty acid composition and inhibits complement (C2) synthesis.

Authors:  R C Strunk; K S Kunke; G B Kolski; B K Revsin
Journal:  Lipids       Date:  1983-07       Impact factor: 1.880

2.  Dephosphorylation of the lipid A moiety of Escherichia coli lipopolysaccharide by mouse macrophages.

Authors:  A A Peterson; R S Munford
Journal:  Infect Immun       Date:  1987-04       Impact factor: 3.441

3.  Expression of the alpha-1-antitrypsin gene in mononuclear phagocytes of normal and alpha-1-antitrypsin-deficient individuals.

Authors:  J F Mornex; A Chytil-Weir; Y Martinet; M Courtney; J P LeCocq; R G Crystal
Journal:  J Clin Invest       Date:  1986-06       Impact factor: 14.808

4.  Uptake and deacylation of bacterial lipopolysaccharides by macrophages from normal and endotoxin-hyporesponsive mice.

Authors:  R S Munford; C L Hall
Journal:  Infect Immun       Date:  1985-05       Impact factor: 3.441

5.  C1 esterase inhibitor gene expression in rat Kupffer cells, peritoneal macrophages and blood monocytes: modulation by interferon gamma.

Authors:  T Armbrust; S Schwögler; G Zöhrens; G Ramadori
Journal:  J Exp Med       Date:  1993-08-01       Impact factor: 14.307

6.  Elastin synthesis by ligamentum nuchae fibroblasts: effects of culture conditions and extracellular matrix on elastin production.

Authors:  R P Mecham; G Lange; J Madaras; B Starcher
Journal:  J Cell Biol       Date:  1981-08       Impact factor: 10.539

  6 in total

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