Enzyme immunoassay for detection of antibodies specific for staphylococcal alpha haemolysin in bovine milk.

Six lactating cows were inoculated in the external inguinal lymph node region with a staphylococcal cell-toxoid preparation. An additional three cows were inoculated with saline as control animals. Enzyme immunoassay (EIA) was used for detection of IgG antibodies in the milk, specific for staphylococcal alpha haemolysin. The positive EIA response in the inoculated cows was followed over an 84 day period. Saline controls showed no response.