Literature DB >> 7016918

Improved method for trypsin digestion of Paraplast sections before immunofluorescence staining.

D Rowse-Eagle, H D Watson, G H Tignor.   

Abstract

Formalin-fixed, Paraplast-embedded tissue sections mounted on subbed slides were digested with crude trypsin in modified Sorensen phosphate buffer containing sodium fluoride before immunofluorescence staining. This method prevented detachment of sections from slides during processing, thereby permitting immunofluorescence and histological examinations of adjacent serial sections.

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Year:  1981        PMID： 7016918      PMCID： PMC273931          DOI： 10.1128/jcm.13.5.996-997.1981

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  2 in total

1.  Enhancement of fluorescent antibody staining of viral antigens in formalin-fixed tissues by trypsin digestion.

Authors:  P T Swoveland; K P Johnson
Journal:  J Infect Dis       Date:  1979-11       Impact factor: 5.226

2.  Diagnosis of rabies by immunofluorescence in trypsin-treated histologic sections.

Authors:  K P Johnson; P T Swoveland; R W Emmons
Journal:  JAMA       Date:  1980-07-04       Impact factor: 56.272
  2 in total
  2 in total

1.  Immunofluorescent staining of trypsinized formalin-fixed brain smears for rabies antigen: results compared with those obtained by standard methods for 221 suspect animal cases in Nigeria.

Authors:  J U Umoh; C D Ezeokoli; A E Okoh
Journal:  J Hyg (Lond)       Date:  1985-02

2.  BLMP-1/Blimp-1 regulates the spatiotemporal cell migration pattern in C. elegans.

Authors:  Tsai-Fang Huang; Chun-Yi Cho; Yi-Ting Cheng; Jheng-Wei Huang; Yun-Zhe Wu; Athena Yi-Chun Yeh; Kiyoji Nishiwaki; Shih-Chung Chang; Yi-Chun Wu
Journal:  PLoS Genet       Date:  2014-06-26       Impact factor: 5.917
  2 in total

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