Literature DB >> 7016915

Comparison of fluorescent-antibody, neutralizing-antibody, and complement-enhanced neutralizing-antibody assays for detection of serum antibody to respiratory syncytial virus.

T N Kaul, R C Welliver, P L Ogra.   

Abstract

A comparison of three assays for the detection of serum antibody to respiratory syncytial virus (RSV) was carried out on 47 serum samples obtained sequentially from infants and young children with RSV infection. Neutralizing-antibody (NA) activity was determined by a semimicromethod of plaque reduction. Complement-enhanced NA activity was determined by the addition of guinea pig complement to NA assays. RSV antibody responses in immunoglobulin G, immunoglobulin M, and immunoglobulin A classes were determined by using indirect immunofluorescence techniques for fluorescent-antibody (FAb) assay. Antibody to RSV was detectable by all three techniques as early as 4 days after the onset of illness. At all phases of illness, titers obtained by complement-enhanced NA assays were significantly greater than those obtained by NA or FAb assays (P less than 0.01). RSV-FAb titers determined in the immunoglobulin G class correlated well with those determined by complement-enhanced NA or NA assays. The data suggest that the FAb assay for detection of RSV antibody in serum is somewhat less sensitive but also less laborious and more rapid than NA assays.

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Year:  1981        PMID： 7016915      PMCID： PMC273923          DOI： 10.1128/jcm.13.5.957-962.1981

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

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Authors:  A L KISCH; K M JOHNSON
Journal:  Proc Soc Exp Biol Med       Date:  1963-03

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Journal:  JAMA       Date:  1961-05-27       Impact factor: 56.272

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Authors:  B Hampar; A L Notkins; M Mage; M A Keehn
Journal:  J Immunol       Date:  1968-03       Impact factor: 5.422

4.  Neutralization of poliovirus by IgA coproantibodies.

Authors:  R Keller; J E Dwyer
Journal:  J Immunol       Date:  1968-08       Impact factor: 5.422

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Authors:  J Mills; J E Van Kirk; P F Wright; R M Chanock
Journal:  J Immunol       Date:  1971-07       Impact factor: 5.422

6.  Effect of heat-labile factors on the neutralization of vaccinia virus by human.

Authors:  K Takabayashi; K McIntosh
Journal:  Infect Immun       Date:  1973-10       Impact factor: 3.441

7.  Shedding of infectious virus/antibody complexes from vesicular lesions of patients with recurrent herpes labialis.

Authors:  C A Daniels; S G LeGoff
Journal:  Lancet       Date:  1975-09-20       Impact factor: 79.321

8.  Complement-enhanced neutralizing antibody response to varicella-zoster virus.

Authors:  C Grose; B J Edmond; P A Brunell
Journal:  J Infect Dis       Date:  1979-04       Impact factor: 5.226

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Authors:  K McIntosh; H B Masters; I Orr; R K Chao; R M Barkin
Journal:  J Infect Dis       Date:  1978-07       Impact factor: 5.226

10.  Effect of complement and viral filtration on the neutralization of respiratory syncytial virus.

Authors:  R H Baughman; J D Fenters; G S Marquis; J C Holper
Journal:  Appl Microbiol       Date:  1968-07
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  7 in total

Review 1.  Detection, pathogenesis, and therapy of respiratory syncytial virus infections.

Authors:  R C Welliver
Journal:  Clin Microbiol Rev       Date:  1988-01       Impact factor: 26.132

2.  Rapid diagnosis of respiratory syncytial virus infection by antigen immunofluorescence detection with monoclonal antibodies and immunoglobulin M immunofluorescence test.

Authors:  Z Kadi; S Dali; S Bakouri; A Bouguermouh
Journal:  J Clin Microbiol       Date:  1986-12       Impact factor: 5.948

3.  Development of antibody-dependent cell-mediated cytotoxicity in the respiratory tract after natural infection with respiratory syncytial virus.

Authors:  T N Kaul; R C Welliver; P L Ogra
Journal:  Infect Immun       Date:  1982-08       Impact factor: 3.441

4.  Interaction of polymorphonuclear leukocytes and viruses in humans: adherence of polymorphonuclear leukocytes to respiratory syncytial virus-infected cells.

Authors:  H Faden; J J Hong; P L Ogra
Journal:  J Virol       Date:  1984-10       Impact factor: 5.103

5.  Virus-induced complement activation and neutrophil-mediated cytotoxicity against respiratory syncytial virus (RSV).

Authors:  T N Kaul; H Faden; R Baker; P L Ogra
Journal:  Clin Exp Immunol       Date:  1984-06       Impact factor: 4.330

Review 6.  Fc-Mediated Antibody Effector Functions During Respiratory Syncytial Virus Infection and Disease.

Authors:  Elisabeth A van Erp; Willem Luytjes; Gerben Ferwerda; Puck B van Kasteren
Journal:  Front Immunol       Date:  2019-03-22       Impact factor: 7.561

7.  Appearance of complement components and immunoglobulins on nasopharyngeal epithelial cells following naturally acquired infection with respiratory syncytial virus.

Authors:  T N Kaul; R C Welliver; P L Ogra
Journal:  J Med Virol       Date:  1982       Impact factor: 2.327
  7 in total

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