Literature DB >> 7013121

A simple method for fusing human lymphocytes with rodent cells in monolayer by polyethylene glycol.

C Brahe, A Serra.   

Abstract

Human lymphocytes were efficiently hybridized with three rodent cell lines growing attached to a substrate, by a procedure similar to the monolayer fusion technique. Lymphocytes in suspension were directly added to the monolayer and cocultured for a few hours. Treatment with polyethylene glycol (PEG) induced close contact between the two cell types, and extensive cell fusion occurred. The rate of hybridization differed markedly for the different cell lines used.

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Year:  1981        PMID： 7013121     DOI： 10.1007/bf01544752

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  6 in total

1.  Rett syndrome: exclusion mapping following the hypothesis of germinal mosaicism for new X-linked mutations.

Authors:  N Archidiacono; M Lerone; M Rocchi; M Anvret; T Ozcelik; U Francke; G Romeo
Journal:  Hum Genet       Date:  1991-04       Impact factor: 4.132

2.  Construction of a map of chromosome 16 by using radiation hybrids.

Authors:  I Ceccherini; G Romeo; S Lawrence; M H Breuning; P C Harris; H Himmelbauer; A M Frischauf; G R Sutherland; G G Germino; S T Reeders
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

3.  Mapping of X-linked Becker muscular dystrophy through crossovers identified by DNA polymorphisms and by haplotype characterization in somatic cell hybrids.

Authors:  L Roncuzzi; S Fadda; M Mochi; L Prosperi; S Sangiorgi; R Santamaria; D Sbarra; D Besana; L Morandi; M Rocchi
Journal:  Am J Hum Genet       Date:  1985-03       Impact factor: 11.025

4.  Characterization of human hybridomas secreting antibody to tetanus toxoid.

Authors:  J W Larrick; K E Truitt; A A Raubitschek; G Senyk; J C Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1983-10       Impact factor: 11.205

5.  Assignment of the catechol-O-methyltransferase gene to human chromosome 22 in somatic cell hybrids.

Authors:  C Brahe; P Bannetta; P Meera Khan; F Arwert; A Serra
Journal:  Hum Genet       Date:  1986-11       Impact factor: 4.132

6.  Acquisition of high metastatic capacity after in vitro fusion of a nonmetastatic tumor line with a bone marrow-derived macrophage.

Authors:  L Larizza; V Schirrmacher; E Pflüger
Journal:  J Exp Med       Date:  1984-11-01       Impact factor: 14.307
  6 in total

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