Literature DB >> 7012125

Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli.

S Brown, E R Brickman, J Beckwith.   

Abstract

We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.

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Year:  1981        PMID： 7012125      PMCID： PMC217102          DOI： 10.1128/jb.146.1.422-425.1981

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  Conditionally lethal amber mutations in the dnaA region of the Escherichia coli chromosome that affect chromosome replication.

Authors:  M Kimura; T Miki; S Hiraga; T Nagata; T Yura
Journal:  J Bacteriol       Date:  1979-12       Impact factor: 3.490

2.  Fusions of the lac operon to the transfer RNA gene tyrT of Escherichia coli.

Authors:  M L Berman; J Beckwith
Journal:  J Mol Biol       Date:  1979-05-25       Impact factor: 5.469

3.  Amber mutations in Escherichia coli essential genes: isolation of mutants affected in the ribosomes.

Authors:  G Delcuve; T Cabezón; A Ghysen; A Herzog; A Bollen
Journal:  Mol Gen Genet       Date:  1977-11-29

4.  Transcription termination at the end of the tryptophan operon of Escherichia coli.

Authors:  L P Guarente; D H Mitchell; J Beckwith
Journal:  J Mol Biol       Date:  1977-05-25       Impact factor: 5.469

5.  Isolation and characterization of a glucosamine-requiring mutant of Escherichia coli K-12 defective in glucosamine-6-phosphate synthetase.

Authors:  H C Wu; T C Wu
Journal:  J Bacteriol       Date:  1971-02       Impact factor: 3.490

6.  Biogenesis of membrane lipids: mutants of Escherichia coli with temperature-sensitive phosphatidylserine decarboxylase.

Authors:  E Hawrot; E P Kennedy
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

7.  Transcription termination factor rho activity is altered in Escherichia coli with suA gene mutations.

Authors:  J P Richardson; C Grimley; C Lowery
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

8.  Localized mutagenesis of any specific small region of the bacterial chromosome.

Authors:  J S Hong; B N Ames
Journal:  Proc Natl Acad Sci U S A       Date:  1971-12       Impact factor: 11.205

9.  Isolation and characterization of conditional-lethal rho mutants of Escherichia coli.

Authors:  H Inoko; K Shigesada; M Imai
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

10.  Specialized transduction of colicin E1 DNA in Escherichia coli K-12.

Authors:  Y Fukumaki; K Shimada; Y Takagi
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205
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  4 in total

1.  Characterization of the detachable Rho-dependent transcription terminator of the fimE gene in Escherichia coli K-12.

Authors:  Paul Hinde; Padraig Deighan; Charles J Dorman
Journal:  J Bacteriol       Date:  2005-12       Impact factor: 3.490

Review 2.  SecA protein: autoregulated initiator of secretory precursor protein translocation across the E. coli plasma membrane.

Authors:  D B Oliver; R J Cabelli; G P Jarosik
Journal:  J Bioenerg Biomembr       Date:  1990-06       Impact factor: 2.945

3.  Identification of a Salmonella typhimurium invasion locus by selection for hyperinvasive mutants.

Authors:  C A Lee; B D Jones; S Falkow
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-01       Impact factor: 11.205

4.  Identification of five new essential genes involved in the synthesis of a secreted protein in Escherichia coli.

Authors:  D B Oliver
Journal:  J Bacteriol       Date:  1985-01       Impact factor: 3.490
  4 in total

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