Literature DB >> 7011404

Purification of an acidic alpha-D-mannosidase from Aspergillus saitoi and specific cleavage of 1,2-alpha-D-mannosidic linkage in yeast mannan.

E Ichishima, M Arai, Y Shigematsu, H Kumagai, R Sumida-Tanaka.   

Abstract

An acidic alpha-D-mannosidase (alpha-D-mannoside mannohydrolase, EC 3.2.1.24) has been isolated from culture filtrate of Aspergillus saitoi. The extracellular alpha-mannosidase was homogeneous in polyacrylamide gel electrophoresis. The molecular weight of the enzyme was 51 000 and the isoelectric point pH 4.5. The purified enzyme has a pH optimum of 5.0, a Km of 0.45 mM with baker's yeast mannan and has no activity towards p-nitrophenyl-alpha-D-mannoside. The mode of action of the enzyme has been studied with baker's yeast mannan and saké yeast mannan. The enzyme cleaves specifically the 1,2-alpha-linked side chain, producing free mannose.

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Year:  1981        PMID： 7011404     DOI： 10.1016/0005-2744(81)90248-5

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  13 in total

1.  Filamentous fungus Aspergillus oryzae has two types of alpha-1,2-mannosidases, one of which is a microsomal enzyme that removes a single mannose residue from Man9GlcNAc2.

Authors:  T Yoshida; Y Kato; Y Asada; T Nakajima
Journal:  Glycoconj J       Date:  2000-11       Impact factor: 2.916

2.  Molecular and enzymic properties of recombinant 1, 2-alpha-mannosidase from Aspergillus saitoi overexpressed in Aspergillus oryzae cells.

Authors:  E Ichishima; N Taya; M Ikeguchi; Y Chiba; M Nakamura; C Kawabata; T Inoue; K Takahashi; T Minetoki; K Ozeki; C Kumagai; K Gomi; T Yoshida; T Nakajima
Journal:  Biochem J       Date:  1999-05-01       Impact factor: 3.857

3.  Dolichol is not a necessary moiety for lipid-linked oligosaccharide substrates of the mannosyltransferases involved in in vitro N-linked-oligosaccharide assembly.

Authors:  I B Wilson; M C Webberley; L Revers; S L Flitsch
Journal:  Biochem J       Date:  1995-09-15       Impact factor: 3.857

4.  Regulation of the protein glycosylation pathway in yeast: structural control of N-linked oligosaccharide elongation.

Authors:  P K Gopal; C E Ballou
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

5.  Antibodies against Manalpha1,2-Manalpha1,2-Man oligosaccharide structures recognize envelope glycoproteins from HIV-1 and SIV strains.

Authors:  Robert J Luallen; Caroline Agrawal-Gamse; Hu Fu; David F Smith; Robert W Doms; Yu Geng
Journal:  Glycobiology       Date:  2009-11-17       Impact factor: 4.313

6.  1,2-alpha-D-mannosidase from Penicillium citrinum: molecular and enzymic properties of two isoenzymes.

Authors:  T Yoshida; T Inoue; E Ichishima
Journal:  Biochem J       Date:  1993-03-01       Impact factor: 3.857

7.  A yeast glycoprotein shows high-affinity binding to the broadly neutralizing human immunodeficiency virus antibody 2G12 and inhibits gp120 interactions with 2G12 and DC-SIGN.

Authors:  Robert J Luallen; Hu Fu; Caroline Agrawal-Gamse; Innocent Mboudjeka; Wei Huang; Fang-Hua Lee; Lai-Xi Wang; Robert W Doms; Yu Geng
Journal:  J Virol       Date:  2009-03-04       Impact factor: 5.103

8.  Chemical modification of Penicillium 1,2-alpha-D-mannosidase by water-soluble carbodi-imide: identification of a catalytically important aspartic acid residue.

Authors:  T Yoshida; K Maeda; M Kobayashi; E Ichishima
Journal:  Biochem J       Date:  1994-10-01       Impact factor: 3.857

9.  The carbohydrate moiety of the acid carboxypeptidase from Aspergillus saitoi.

Authors:  Y Chiba; Y Yamagata; S Iijima; T Nakajima; E Ichishima
Journal:  Curr Microbiol       Date:  1993-11       Impact factor: 2.188

10.  An engineered Saccharomyces cerevisiae strain binds the broadly neutralizing human immunodeficiency virus type 1 antibody 2G12 and elicits mannose-specific gp120-binding antibodies.

Authors:  Robert J Luallen; Jianqiao Lin; Hu Fu; Karen K Cai; Caroline Agrawal; Innocent Mboudjeka; Fang-Hua Lee; David Montefiori; David F Smith; Robert W Doms; Yu Geng
Journal:  J Virol       Date:  2008-04-23       Impact factor: 5.103
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