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Literature DB >> 7009552

Construction and characterization of hybrid plasmids containing the Escherichia coli nrd region.

Abstract

Recombinant plasmids containing all or part of the genetic region of Escherichia coli coding for the two subunits of ribonucleoside diphosphate reductase (proteins B1 and B2) were constructed with the aid of the multicopy plasmid pBR322. Two of these plasmids (pPS1 and pPS2) appeared to carry both a regulator and the complete structural information for the enzyme and, after transformation of E. coli, directed a 10- to 20-fold overproduction of both proteins B1 and B2. The other plasmids (pPS101 and pPS201) carried structural information for only protein B2. Cells carrying pPS1 and pPS2 showed a 5- to 500-fold increased resistance against the drug hydroxyurea. This establishes that in E. coli the inhibition of deoxyribonucleic acid synthesis by hydroxyurea is fully explained by its action on ribonucleotide reductase.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1980 PMID： 7009552 PMCID： PMC294315 DOI： 10.1128/jb.143.2.561-568.1980

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

33 in total

1. A mutant of Escherichia coli defective in ribonucleosidediphosphate reductase. 2. Characterization of the enzymatic defect.

Authors: J A Fuchs; H O Karlström
Journal: Eur J Biochem Date: 1973-02-01

2. Defective gene product in dnaF mutant of Escherichia coli.

Authors: J A Fuchs; H O Karlström; H R Warner; P Reichard
Journal: Nat New Biol Date: 1972-07-19

3. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors: D B Clewell
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

4. Electron spin resonance of the iron-containing protein B2 from ribonucleotide reductase.

Authors: A Ehrenberg; P Reichard
Journal: J Biol Chem Date: 1972-06-10 Impact factor: 5.157

5. Turnover of the deoxyribonucleoside triphosphates in Escherichia coli 15 T during thymine starvation.

Authors: J Neuhard; E Thomassen
Journal: Eur J Biochem Date: 1971-05-11

6. Inhibition of ribonucleoside diphosphate reductase by hydroxyurea.

Authors: I H Krakoff; N C Brown; P Reichard
Journal: Cancer Res Date: 1968-08 Impact factor: 12.701

7. Genetic control of L-alpha-glycerophosphate system in Escherichia coli.

Authors: N R Cozzarelli; W B Freedberg; E C Lin
Journal: J Mol Biol Date: 1968-02-14 Impact factor: 5.469

8. Ribonucleoside diphosphate reductase. Purification of the two subunits, proteins B1 and B2.

Authors: N C Brown; Z N Canellakis; B Lundin; P Reichard; L Thelander
Journal: Eur J Biochem Date: 1969-07

9. Studies with hydroxyurea. I. The reversible inhibition of bacterial DNA synthesis and the effect of hydroxyurea on the bactericidal action of streptomycin.

Authors: H S Rosenkranz; A J Garro; J A Levy; H S Carr
Journal: Biochim Biophys Acta Date: 1966-03-21

10. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors: S N Cohen; A C Chang; L Hsu
Journal: Proc Natl Acad Sci U S A Date: 1972-08 Impact factor: 11.205

25 in total

1. Interactions of glutaredoxins, ribonucleotide reductase, and components of the DNA replication system of Escherichia coli.

Authors: Ron Ortenberg; Stéphanie Gon; Amir Porat; Jon Beckwith
Journal: Proc Natl Acad Sci U S A Date: 2004-04-27 Impact factor: 11.205

10. Alterations in intracellular deoxyribonucleotide levels of mutationally altered ribonucleotide reductases in Escherichia coli.

Authors: A Platz; M Karlsson; S Hahne; S Eriksson; B M Sjöberg
Journal: J Bacteriol Date: 1985-12 Impact factor: 3.490

Construction and characterization of hybrid plasmids containing the Escherichia coli nrd region.

1. A mutant of Escherichia coli defective in ribonucleosidediphosphate reductase. 2. Characterization of the enzymatic defect.

2. Defective gene product in dnaF mutant of Escherichia coli.

3. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

4. Electron spin resonance of the iron-containing protein B2 from ribonucleotide reductase.

5. Turnover of the deoxyribonucleoside triphosphates in Escherichia coli 15 T during thymine starvation.

6. Inhibition of ribonucleoside diphosphate reductase by hydroxyurea.

7. Genetic control of L-alpha-glycerophosphate system in Escherichia coli.

8. Ribonucleoside diphosphate reductase. Purification of the two subunits, proteins B1 and B2.

9. Studies with hydroxyurea. I. The reversible inhibition of bacterial DNA synthesis and the effect of hydroxyurea on the bactericidal action of streptomycin.

10. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

1. Interactions of glutaredoxins, ribonucleotide reductase, and components of the DNA replication system of Escherichia coli.

Review 2. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

3. Measurement of in vivo expression of nrdA and nrdB genes of Escherichia coli by using lacZ gene fusions.

4. Regulation of the operon encoding ribonucleotide reductase: role of the negative sites in nrd repression.

5. Genetic and morphological characterization of ftsB and nrdB mutants of Escherichia coli.

6. Characterization of the ftsB gene as an allele of the nrdB gene in Escherichia coli.

7. Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase.

8. Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase.

9. Primary structure of the Escherichia coli ribonucleoside diphosphate reductase operon.

10. Alterations in intracellular deoxyribonucleotide levels of mutationally altered ribonucleotide reductases in Escherichia coli.