Literature DB >> 7009196

Immobilized subunits can function as an affinity sorbent to purify oligomeric enzymes: the usefulness of hybridization on the solid support.

V I Muronetz, L I Ashmarina, N K Nagradova.   

Abstract

Immobilized dimers of yeast glyceraldehyde-3-phosphate dehydrogenase covalently bound to sepharose were shown to form hybrids with soluble dimers of the homologous enzymes present in crude tissue extracts (rats skeletal muscle, rat, rabbit and bovine hearts, rat liver, rat brain). Immobilized hybrid tetramers were then dissociated to form purified soluble enzymes.

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Year:  1981        PMID： 7009196     DOI： 10.1007/bf01965542

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  8 in total

1.  Yeast Glyceraldehyde-3-phosphate dehydrogenase. I. Electrophoresis of fractions precipitated by nucleic acid.

Authors:  E G KREBS
Journal:  J Biol Chem       Date:  1953-02       Impact factor: 5.157

2.  Cold inactivation of glyceraldehyde-phosphate dehydrogenase from rat skeletal muscle.

Authors:  N K Nagradova; V I Muronetz; I D Grozdova; T O Golovina
Journal:  Biochim Biophys Acta       Date:  1975-01-23

3.  Dissociation and reassociation of immobilized porphobilinogen synthase: use of immobilized subunits for enzyme isolation.

Authors:  D Gurne; J Chen; D Shemin
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

4.  Immobilized hybrids of glyceraldehyde-3-phosphate dehydrogenase.

Authors:  V I Muronetz; R A Asryants; N K Nagradova
Journal:  Biochim Biophys Acta       Date:  1978-07-07

5.  Immobilized dimers of D-glyceraldehyde-3-phosphate dehydrogenase.

Authors:  N K Nagradova; T O Golovina; A T Mevkh
Journal:  FEBS Lett       Date:  1974-12-15       Impact factor: 4.124

6.  Influence of substrates on the dissociation of rabbit muscle D-glyceraldehyde 3-phosphate dehydrogenase.

Authors:  V D Hoagland; D C Teller
Journal:  Biochemistry       Date:  1969-02       Impact factor: 3.162

7.  Half-of-the-sites reactivity of rat skeletal muscle D-glyceraldehyde-3-phosphate dehydrogenase.

Authors:  T O Golovina; V I Muronetz; N K Nagradova
Journal:  Biochim Biophys Acta       Date:  1978-05-11

8.  A convenient method for the estimation of Sepharose-bound protein.

Authors:  T O Golovina; T V Cherednikova; A T Mevkh; N K Nagradova
Journal:  Anal Biochem       Date:  1977-12       Impact factor: 3.365
  8 in total

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