Synthesis of Saccharomyces cerevisiae catalase A in vitro.

mRNA isolated from cells of the yeast Saccharomyces cerevisiae was translated in the cell-free protein synthesis system from wheat germ. Catalase proteins synthesized were isolated from incubation mixtures by immunoadsorption followed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. On dodecyl sulfate gels catalase A synthesized by the wheat germ system migrates like catalase A protein synthesized in vivo. Evidence is presented that yeast catalase T and A synthesized in vivo are no glycoproteins. Synthesis of the two catalase proteins in the wheat germ system and dissimilarity of proteolytic fingerprints of the two proteins demonstrate conclusively that catalase T and A are biogenetically unrelated.