Literature DB >> 7005891

[Rapid method for determining protein concentrations in the cells of microorganisms].

I A Gorina, V I Iakovleva.   

Abstract

The method of Lowry as modified by Peterson (who had changed concentrations of reagents and added a new reagent, sodium dodecyl sulfate) has been tested. It has been shown that the modified method can be applied for rapid measurement of protein in the suspension of bacterial cells. The effect of the following compounds on the protein estimation by the modified method in bovine serum albumin has been studied: potassium chloride, potassium phosphate, sucrose, EDTA, tris, ammonium acetate, Triton X-100, tyrosine, phenol, glycerol, 2-mercaptoethanol.

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Year:  1980        PMID: 7005891

Source DB:  PubMed          Journal:  Prikl Biokhim Mikrobiol        ISSN: 0555-1099


  1 in total

1.  Kinetics of growth and metabolism of Clostridium thermosaccharolyticum culture. Isolation and characteristics of its plasmids.

Authors:  N G Belogurova; T P Mosolova; S V Kalyuzhnyy; S D Varfolomeyev
Journal:  Appl Biochem Biotechnol       Date:  1991-01       Impact factor: 2.926

  1 in total

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