Literature DB >> 7003151

Membrane transport of p-nitrophenyl-alpha-galactoside by the melibiose carrier of Escherichia coli.

K Ottina, J Lopilato, T H Wilson.   

Abstract

p-Nitrophenyl-alpha-galactoside (alpha-pNPG) was found to be a substrate for the melibiose transport system of Escherichia coli. This sugar enters induced cells via the carrier and is split by alpha-galactosidase to galactose and p-nitrophenol. In mutant cells lacking the alpha-galactosidase [3H]-alpha-pNPG accumulated to concentrations 15 times higher than the external medium. The transport of alpha-pNPG is inhibited by both Na+ and Li+. Na+ (10 mM) reduced the Km for alpha-pNPG from 0.45 to 0.18 mM and reduced the Vmax from 6.7 nmoles/min/mg dry wt to a value of 3.0.

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Year:  1980        PMID： 7003151     DOI： 10.1007/bf01875968

Source DB:  PubMed          Journal:  J Membr Biol        ISSN： 0022-2631            Impact factor:   1.843


  13 in total

1.  Sodium-dependent methyl 1-thio-beta-D-galactopyranoside transport in membrane vesicles isolated from Salmonella typhimurium.

Authors:  H Tokuda; H R Kaback
Journal:  Biochemistry       Date:  1977-05-17       Impact factor: 3.162

2.  RESTORATION OF OPERON ACTIVITY BY SUPPRESSORS.

Authors:  J BECKWITH
Journal:  Biochim Biophys Acta       Date:  1963-09-17

3.  [Not Available].

Authors:  G N COHEN; H V RICKENBERG
Journal:  Ann Inst Pasteur (Paris)       Date:  1956-11

4.  Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli.

Authors:  J Lopilato; T Tsuchiya; T H Wilson
Journal:  J Bacteriol       Date:  1978-04       Impact factor: 3.490

5.  Dierect measurement of the binding of labeled sugars to the lactose permease M protein.

Authors:  E P Kennedy; M K Rumley; J B Armstrong
Journal:  J Biol Chem       Date:  1974-01-10       Impact factor: 5.157

6.  Alpha-galactosidase activity in cell-free extracts of Escherichia coli.

Authors:  R Schmitt; B Rotman
Journal:  Biochem Biophys Res Commun       Date:  1966-03-08       Impact factor: 3.575

7.  The alpha-galactosidase from Escherichia coli K12.

Authors:  C Burstein; A Kepes
Journal:  Biochim Biophys Acta       Date:  1971-01-26

8.  A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

Authors:  L Clarke; J Carbon
Journal:  Cell       Date:  1976-09       Impact factor: 41.582

9.  A sodium-dependent sugar co-transport system in bacteria.

Authors:  J Stock; S Roseman
Journal:  Biochem Biophys Res Commun       Date:  1971-07-02       Impact factor: 3.575

10.  Effect of lithium ion on melibiose transport in Escherichia coli.

Authors:  T Tsuchiya; J Lopilato; T H Wilson
Journal:  J Membr Biol       Date:  1978-07-21       Impact factor: 1.843
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