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Literature DB >> 7001366

On the promoter complex formation rate of E. coli RNA polymerases with T7 phage DNA.

B N Belintsev, S K Zavriev, M F Shemyakin.

Abstract

Influence of ionic strength on the kinetics of the promoter complex formation between E. coli RNA polymerase and T7 phage DNA was investigated using a membrane filter assay. The enzyme-promoter association rate constant was determined. It varies from 10(9) to 3 x 10(7) M-1 sec-1 when the ionic strength is changed from zero to 0.15 M NaCl. Basing on the theoretical analysis of experimental data obtained the model for the promoter site selection assuming the enzyme sliding along the DNA is discussed.

Entities: Chemical Species

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Year: 1980 PMID： 7001366 PMCID： PMC323999 DOI： 10.1093/nar/8.6.1391

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

22 in total

1. Electron microscopic localization of the binding sites of Escherichia coli RNA polymerase in the early promoter region of T7 DNA.

Authors: C Bordier; J Dubochet
Journal: Eur J Biochem Date: 1974-05-15

2. Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. I. An assay for the rate and extent of ribonucleic acid chain initiation.

Authors: W F Mangel; M J Chamberlin
Journal: J Biol Chem Date: 1974-05-25 Impact factor: 5.157

3. Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. II. The effect of alterations in ionic strength of chain initiation and on the conformation of binary complexes.

Authors: W F Mangel; M J Chamberlin
Journal: J Biol Chem Date: 1974-05-25 Impact factor: 5.157

7. An adenosine triphosphate-dependent deoxyribonuclease from Hemophilus influenzae Rd. I. Purification and properties of the enzyme.

Authors: E A Friedman; H O Smith
Journal: J Biol Chem Date: 1972-05-10 Impact factor: 5.157

On the promoter complex formation rate of E. coli RNA polymerases with T7 phage DNA.

1. Electron microscopic localization of the binding sites of Escherichia coli RNA polymerase in the early promoter region of T7 DNA.

2. Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. I. An assay for the rate and extent of ribonucleic acid chain initiation.

3. Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. II. The effect of alterations in ionic strength of chain initiation and on the conformation of binary complexes.

4. Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection.

5. Studies of the binding of Escherichia coli RNA polymerase to DNA. II. The kinetics of the binding reaction.

6. RNA polymerase-DNA complexes. 3. Binding of actinomycin D to RNA polymerase-DNA complex.

7. An adenosine triphosphate-dependent deoxyribonuclease from Hemophilus influenzae Rd. I. Purification and properties of the enzyme.

8. Studies of the binding of actinomycin and related compounds to DNA.

9. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.

10. The 5'-terminal nucleotides of T7 bacteriophage deoxyribonucleic acid.

Review 1. Single-molecule studies of RNA polymerase: motoring along.

2. Facilitated diffusion of a DNA binding protein on chromatin.