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Literature DB >> 6999805

Serodiagnosis of human leptospirosis by enzyme-linked-immunosorrbent-assay (ELISA).

W J Terpstra, G S Ligthart, G J Schoone.

Abstract

An enzyme-linked-immunosorbent-assay (ELISA) is described for the serodiagnosis of leptospirosis. Using an antigen prepared from a heated culture of a single leptospira strain (Wijnberg) the ELISA is a genusspecific test. The microscopic agglutination test (MAT) served as a reference. ELISA and MAT results agreed in 95% of the sera from 96 leptospirosis patients. One false positive was found in 217 controls. The ELISA is sensitive, specific and relatively easy to perform.

Entities: Disease Species

Mesh：

Substances：
Antibodies, Bacterial

Year: 1980 PMID： 6999805

Source DB: PubMed Journal: Zentralbl Bakteriol A ISSN： 0172-5599

Keyword Cloud
Cited

28 in total

1. Lateral-flow assay for rapid serodiagnosis of human leptospirosis.

Authors: H L Smits; C K Eapen; S Sugathan; M Kuriakose; M H Gasem; C Yersin; D Sasaki; B Pujianto; M Vestering; T H Abdoel; G C Gussenhoven
Journal: Clin Diagn Lab Immunol Date: 2001-01

Review 2. Leptospirosis.

Authors: P N Levett
Journal: Clin Microbiol Rev Date: 2001-04 Impact factor: 26.132

3. An evaluation of three commercial kits for use as screening methods for the detection of leptospiral antibodies in the UK.

Authors: W J Zochowski; M F Palmer; T J Coleman
Journal: J Clin Pathol Date: 2001-01 Impact factor: 3.411

4. Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis.

Authors: Mary D Bajani; David A Ashford; Sandra L Bragg; Christopher W Woods; Tin Aye; Richard A Spiegel; Brian D Plikaytis; Bradley A Perkins; Maureen Phelan; Paul N Levett; Robbin S Weyant
Journal: J Clin Microbiol Date: 2003-02 Impact factor: 5.948

5. Randomised primary health center based interventions to improve the diagnosis and treatment of undifferentiated fever and dengue in Vietnam.

Authors: Hoang L Phuong; Tran T T Nga; Phan T Giao; Le Q Hung; Tran Q Binh; Nguyen V Nam; Nico Nagelkerke; Peter J de Vries
Journal: BMC Health Serv Res Date: 2010-09-21 Impact factor: 2.655

Serodiagnosis of human leptospirosis by enzyme-linked-immunosorrbent-assay (ELISA).

1. Lateral-flow assay for rapid serodiagnosis of human leptospirosis.

Review 2. Leptospirosis.

3. An evaluation of three commercial kits for use as screening methods for the detection of leptospiral antibodies in the UK.

4. Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis.

5. Randomised primary health center based interventions to improve the diagnosis and treatment of undifferentiated fever and dengue in Vietnam.

6. Leptospiral antibodies in patients from a Barbadian general practice.

7. Two methods for rapid serological diagnosis of acute leptospirosis.

8. Development of an indirect enzyme-linked immunosorbent assay for the detection of leptospiral antibodies in dogs.

9. Serological interrelationship of Leptospira serovar and genus-specific antigens by enzyme-linked immunosorbent assay.

10. Murine typhus and leptospirosis as causes of acute undifferentiated fever, Indonesia.