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Literature DB >> 6999016

Monoclonal antibodies specific for dengue virus type 3.

Abstract

Mouse lymphocyte hybridomas were prepared by polyethylene glycol-mediated fusion of cells from a mouse plasmacytoma line with lymphocytes from a mouse hyperimmunized with dengue virus type 3 (dengue-3). Media from 50 hybrid colonies were screened; 46 of them showed antibody activity against dengue-3-infected cells as determined by an indirect immunofluorescent antibody technique. Dengue monoclonal antibody obtained after cloning one of these colonies demonstrated activity in hemagglutination inhibition and indirect immunofluorescent antibody assays with dengue-3 antigen, but not type 1, 2, and 4 antigens. In addition, this antibody activity could be removed from culture media only by absorption with dengue-3 antigen.

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Year: 1980 PMID： 6999016 PMCID： PMC273524 DOI： 10.1128/jcm.12.1.74-78.1980

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

17 in total

1. Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors: G Köhler; C Milstein
Journal: Nature Date: 1975-08-07 Impact factor: 49.962

Review 2. Monoclonal antibodies. Hybrid myelomas--a revolution in serology and immunogenetics.

Authors: R G Kennett
Journal: Am J Hum Genet Date: 1979-09 Impact factor: 11.025

3. Identification of dengue viruses using complement fixing antigen produced in mosquitoes.

Authors: T T Kuberski; L Rosen
Journal: Am J Trop Med Hyg Date: 1977-05 Impact factor: 2.345

4. A simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence.

Authors: T T Kuberski; L Rosen
Journal: Am J Trop Med Hyg Date: 1977-05 Impact factor: 2.345

5. Comparison of dengue-2 and dengue-3 virus strains by neutralization tests and identification of a subtype of dengue-3.

Authors: P K Russell; J M McCown
Journal: Am J Trop Med Hyg Date: 1972-01 Impact factor: 2.345

6. Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing.

Authors: D W Trent
Journal: J Virol Date: 1977-06 Impact factor: 5.103

7. Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells.

Authors: R H Kennett; K A Denis; A S Tung; N R Klinman
Journal: Curr Top Microbiol Immunol Date: 1978 Impact factor: 4.291

8. A better cell line for making hybridomas secreting specific antibodies.

Authors: M Shulman; C D Wilde; G Köhler
Journal: Nature Date: 1978-11-16 Impact factor: 49.962

9. Immunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies.

Authors: D Dittmar; T J Cleary; A Castro
Journal: J Clin Microbiol Date: 1979-04 Impact factor: 5.948

10. Morphological, chemical, and biological characterization of Japanese encephalitis virus virion and its hemagglutinin.

Authors: T Kitano; K Suzuki; T Yamaguchi
Journal: J Virol Date: 1974-09 Impact factor: 5.103

7 in total

Review 1. Monoclonal antibodies against microorganisms.

Authors: R A Polin
Journal: Eur J Clin Microbiol Date: 1984-10 Impact factor: 3.267

2. Topographical analysis of antigenic determinants on envelope glycoprotein V3 (E) of Japanese encephalitis virus, using monoclonal antibodies.

Authors: J Kimura-Kuroda; K Yasui
Journal: J Virol Date: 1983-01 Impact factor: 5.103