New translocation sequence mediating tetracycline resistance found in Escherichia coli pathogenic for piglets.

A discrete piece of deoxyribonucleic acid coding for tetracycline (Tc) resistance was found to move from one R plasmid to another in an Escherichia coli strain which is pathogenic for piglets. Since this phenomenon took place also in rec strains, the Tc segment was classified as a transposon and called Tn804. Restriction enzyme analysis with EcoRI, BglII, and HindIII indicated that Tn804 is related to Tn10, a well-known transposon that codes for resistance to tetracycline. Hybridization between plasmids carrying the two transposons provided proof of homology between Tn10 and prt of Tn804. Electron microscopic studies showed a transposon-like structure composed of one loop-stem structure with inverted repetitions of approximately 0.9 megadaltons inserted into the loop of a second loop-stem structure. It is suggested that Tn804 is composed of Tn10 plus another transposable sequence.