Literature DB >> 6997367

Ultrastructural localization of alpha-OH acid oxidase in peroxisomes with the CeCl3 technique.

G Arnold, E Holtzman.   

Abstract

alpha-OH acid oxidase activity was demonstrated in peroxisomes of glutaraldehyde-fixed tissues using the CeCl3 cytochemical method. For kidney, alpha-OH butyrate or alpha-OH valerate were used as substrates. These substrates gave much less reaction product in liver peroxisomes. Liver peroxisomes were more reactive with glycolate as substrate. Glycolate gave little or no reaction product in kidney peroxisomes. alpha-keto-glutarate inhibited activity of the enzyme with alpha-OH butyrate as substrate.

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Year:  1980        PMID: 6997367     DOI: 10.1177/28.9.6997367

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN: 0022-1554            Impact factor:   2.479


  5 in total

1.  Immunoelectron microscopic localization of D-amino acid oxidase in rat kidney and liver.

Authors:  M E Perotti; E Gavazzi; L Trussardo; N Malgaretti; B Curti
Journal:  Histochem J       Date:  1987-03

2.  Peroxisomes in wild-type and rosy mutant Drosophila melanogaster.

Authors:  M E Beard; E Holtzman
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

Review 3.  In situ heterogeneity of peroxisomal oxidase activities: an update.

Authors:  R J Van den Munckhof
Journal:  Histochem J       Date:  1996-06

4.  Electron microscopic cytochemical localization of alpha-hydroxyacid oxidase in rat kidney cortex. Heterogeneous staining of peroxisomes.

Authors:  S Angermüller; C Leupold; K Zaar; H D Fahimi
Journal:  Histochemistry       Date:  1986

5.  Electron microscopic cytochemical localization of alpha-hydroxyacid oxidase in rat liver. Association with the crystalline core and matrix of peroxisomes.

Authors:  S Angermüller; C Leupold; A Völkl; H D Fahimi
Journal:  Histochemistry       Date:  1986
  5 in total

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