Literature DB >> 6995823

Crossed immunoelectrophoresis and chemical structural analysis used for characterization of two varieties of Escherichia coli K2 polysaccharide antigen.

J C Larsen, F Orskov, I Orskov, M A Schmidt, B Jann, K Jann.   

Abstract

Crossed immunoelectrophoresis has shown that the Escherichia coli polysaccharide K2 antigen exists in two forms, K2ab and K2a. In confirmation of this finding, chemical structural analysis showed that K2ab, in addition to the galactose, glycerol and phosphate groups common to K2ab and K2a, contained 0 acetyl groups. The contrast between the earlier and the present K2 designation is discussed and the superiority of the CIE technique for this type of analysis is underlined.

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Year:  1980        PMID: 6995823     DOI: 10.1007/bf02122853

Source DB:  PubMed          Journal:  Med Microbiol Immunol        ISSN: 0300-8584            Impact factor:   3.402


  14 in total

1.  Studies on a number of Escherichia coli strains belonging to O group 86.

Authors:  F ØRSKOV
Journal:  Acta Pathol Microbiol Scand       Date:  1954

Review 2.  Serology, chemistry, and genetics of O and K antigens of Escherichia coli.

Authors:  I Orskov; F Orskov; B Jann; K Jann
Journal:  Bacteriol Rev       Date:  1977-09

3.  A modified antigen--antibody crossed electrophoresis characterizing the specificity and titre of human precipiting against Candida albicans.

Authors:  J Svendsen; N H Axelsen
Journal:  J Immunol Methods       Date:  1972-01       Impact factor: 2.303

Review 4.  Immunological properties of teichoic acids.

Authors:  K W Knox; A J Wicken
Journal:  Bacteriol Rev       Date:  1973-06

5.  A manual of quantitative immunoelectrophoresis. Methods and applications. 1. General remarks on principles, equipment, reagents and procedures.

Authors:  B Weeke
Journal:  Scand J Immunol Suppl       Date:  1973

6.  What is the nature of E. coli antigens? An attempt to analyse the K-antigens by various serological methods.

Authors:  I Orskov; F Orskov
Journal:  Arch Immunol Ther Exp (Warsz)       Date:  1968       Impact factor: 4.291

7.  [Growth and lipopolysaccharide (O-antigen) levels of salmonella in cultures of agar media].

Authors:  S Schlecht; O Westphal
Journal:  Zentralbl Bakteriol Orig       Date:  1966-06

8.  The structure of the capsular polysaccharide (K5 antigen) of urinary-tract-infective Escherichia coli 010:K5:H4. A polymer similar to desulfo-heparin.

Authors:  W F Vann; M A Schmidt; B Jann; K Jann
Journal:  Eur J Biochem       Date:  1981-05-15

9.  Immunoelectrophoretic patterns of extracts from Escherichia coli O antigen test strains O1 to O157 examinations in homologous OK sera. Further comments on the classification of Escherichia K antigens.

Authors:  F Orskov; I Orskov
Journal:  Acta Pathol Microbiol Scand B Microbiol Immunol       Date:  1972

10.  Form variation in Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide.

Authors:  F Orskov; I Orskov; A Sutton; R Schneerson; W Lin; W Egan; G E Hoff; J B Robbins
Journal:  J Exp Med       Date:  1979-03-01       Impact factor: 14.307

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  2 in total

1.  Characterization of the lipopolysaccharide O antigens of Actinobacillus pleuropneumoniae serotypes 9 and 11: antigenic relationships among serotypes 9, 11, and 1.

Authors:  L M Beynon; D W Griffith; J C Richards; M B Perry
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

2.  Serological, chemical, and structural analyses of the Escherichia coli cross-reactive capsular polysaccharides K13, K20, and K23.

Authors:  W F Vann; T Soderstrom; W Egan; F P Tsui; R Schneerson; I Orskov; F Orskov
Journal:  Infect Immun       Date:  1983-02       Impact factor: 3.441

  2 in total

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