Literature DB >> 6995555

Analysis of rat hemopoietic cells on the fluorescence-activated cell sorter. II. Isolation of terminal deoxynucleotidyl transferase-positive cells.

I Goldschneider, D Metcalf, T Mandel, F J Bollum.   

Abstract

A method is described by which highly enriched populations of viable terminal deoxynucleotidyl transferase-positive (TdT+) cells can be isolated from rat bone marrow by use of the fluorescence-activated cell sorter. Such cells have been postulated to be progenitors of thymocytes and, possibly, of B lymphocytes, and may serve as the targets of neoplastic transformation in acute lymphoblastic leukemia. The separation procedure is based on differences in relative low-angle light scatter and relative fluorescence intensity for Thy-1 antigen between TdT+ cells and other lymphohemopoietic cell populations in bone marrow. Simultaneous sorting of bone marrow cells according to these two parameters resulted in a mean 87% purification of TdT+ cells. The morphological characteristics of the isolated TdT+ cells are described at the light and electron miscroscopic levels.

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Year:  1980        PMID： 6995555      PMCID： PMC2185952          DOI： 10.1084/jem.152.2.438

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  19 in total

1.  Is terminal deoxynucleotidyl transferase a somatic mutagen in lymphocytes?

Authors:  D Baltimore
Journal:  Nature       Date:  1974-03-29       Impact factor: 49.962

2.  Fluorescence activated cell sorting.

Authors:  W A Bonner; H R Hulett; R G Sweet; L A Herzenberg
Journal:  Rev Sci Instrum       Date:  1972-03       Impact factor: 1.523

3.  Demonstration of terminal deoxynucleotidyl transferase in thymocytes by immunofluorescence.

Authors:  I Goldschneider; K E Gregoire; R W Barton; F J Bollum
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

4.  Polycythemia vera: stem-cell and probable clonal origin of the disease.

Authors:  J W Adamson; P J Fialkow; S Murphy; J F Prchal; L Steinmann
Journal:  N Engl J Med       Date:  1976-10-21       Impact factor: 91.245

5.  Antibody to terminal deoxynucleotidyl transferase.

Authors:  F J Bollum
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

6.  Intracellular distribution of terminal deoxynucleotidyl transferase in rat bone marrow and thymus.

Authors:  K E Gregoire; I Goldschneider; R W Barton; F J Bollum
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

7.  Pure and mixed erythroid colony formation in vitro stimulated by spleen conditioned medium with no detectable erythropoietin.

Authors:  G R Johnson; D Metcalf
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

8.  Terminal deoxynucleotidyl transferase is found in prothymocytes.

Authors:  A E Silverstone; H Cantor; G Goldstein; D Baltimore
Journal:  J Exp Med       Date:  1976-08-01       Impact factor: 14.307

9.  Murine terminal deoxynucleotidyl transferase: cellular distribution and response to cortisone.

Authors:  P C Kung; A E Siverstone; R P McCaffrey; D Baltimore
Journal:  J Exp Med       Date:  1975-04-01       Impact factor: 14.307

10.  The identification in adult bone marrow of pluripotent and restricted stem cells of the myeloid and lymphoid systems.

Authors:  S Abramson; R G Miller; R A Phillips
Journal:  J Exp Med       Date:  1977-06-01       Impact factor: 14.307
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  8 in total

1.  Stems and standards: social interaction in the search for blood stem cells.

Authors:  Melinda Bonnie Fagan
Journal:  J Hist Biol       Date:  2010       Impact factor: 1.326

2.  Studies on the differentiation of T lymphocytes in sheep. I. Recognition of a sheep T-lymphocyte differentiation antigen by a monoclonal antibody T-80.

Authors:  M Miyasaka; I Heron; L Dudler; R N Cahill; L Forni; T Knaak; Z Trnka
Journal:  Immunology       Date:  1983-07       Impact factor: 7.397

3.  Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies.

Authors:  J Kandzia; M J Anderson; W Müller-Ruchholtz
Journal:  J Cancer Res Clin Oncol       Date:  1981       Impact factor: 4.553

4.  Induction of terminal deoxynucleotidyl transferase and Lyt antigens with thymosin: identification of multiple subsets of prothymocytes in mouse bone marrow and spleen.

Authors:  I Goldschneider; A Ahmed; F J Bollum; A L Goldstein
Journal:  Proc Natl Acad Sci U S A       Date:  1981-04       Impact factor: 11.205

5.  Phenotype and proliferation of early B lymphocyte precursor cells in mouse bone marrow.

Authors:  Y H Park; D G Osmond
Journal:  J Exp Med       Date:  1987-02-01       Impact factor: 14.307

6.  Analysis of rat hemopoietic cells on the fluorescence-activated cell sorter. I. Isolation of pluripotent hemopoietic stem cells and granulocyte-macrophage progenitor cells.

Authors:  I Goldschneider; D Metcalf; F Battye; T Mandel
Journal:  J Exp Med       Date:  1980-08-01       Impact factor: 14.307

7.  A selective culture system for generating terminal deoxynucleotidyl transferase-positive (TdT+) lymphoid precursor cells in vitro. I. Description of the culture system.

Authors:  J Hayashi; E S Medlock; I Goldschneider
Journal:  J Exp Med       Date:  1984-12-01       Impact factor: 14.307

8.  Identification of thymocyte progenitors in hemopoietic tissues of the rat. II. Enrichment of functional prothymocytes on the fluorescence-activated cell sorter.

Authors:  D L Greiner; I Goldschneider; R W Barton
Journal:  J Exp Med       Date:  1982-11-01       Impact factor: 14.307
  8 in total

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