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Literature DB >> 6995494

Detection of virus particles by electron microscopy with polyacrylamide hydrogel.

Abstract

The use of lyphogel to concentrate the number of virus particles in specimens for electron microscopic examination was studied in parallel with ultracentrifugation. One hundred faecal and urine samples were compared. Both methods had a similar sensitivity. Lyphogel was economical, simple, and rapid in use; in contrast to ultracentrifugation, it required relatively little material. The procedure could be done within a safety cabinet, and virus particles were morphologically undamaged by the process.

Entities: Chemical

Mesh：

Substances：
Gels

Year: 1980 PMID： 6995494 PMCID： PMC1146115 DOI： 10.1136/jcp.33.5.484

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

7 in total

Review 1. Viruses in the stools.

Authors: C R Madeley
Journal: J Clin Pathol Date: 1979-01 Impact factor: 3.411

2. Comparison of small hepatitis B surface antigen particles and human serum low density lipoprotein molecules by electron microscopy.

Authors: M H Solaas
Journal: Acta Pathol Microbiol Scand B Date: 1978-06

3. Diagnostic electron microscopy of faeces. I. The viral flora of the faeces as seen by electron microscopy.

Authors: T H Flewett; A S Bryden; H Davies
Journal: J Clin Pathol Date: 1974-08 Impact factor: 3.411

4. Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis.

Authors: R F Bishop; G P Davidson; I H Holmes; B J Ruck
Journal: Lancet Date: 1974-02-02 Impact factor: 79.321

5. Density in cesium chloride of the 27 nm "8FIIa" particle associated with acute infectious nonbacterial gastroenteritis: determination by ultra-centrifugation and immune electron microscopy.

Authors: A Z Kapikian; J L Gerin; R G Wyatt; T S Thornhill; R M Chanock
Journal: Proc Soc Exp Biol Med Date: 1973-03

6. Hepatitis-associated antigen: improved sensitivity in detection.

Authors: M Ashcavai; R L Peters
Journal: Am J Clin Pathol Date: 1971-03 Impact factor: 2.493

7. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis.

Authors: A Z Kapikian; R G Wyatt; R Dolin; T S Thornhill; A R Kalica; R M Chanock
Journal: J Virol Date: 1972-11 Impact factor: 5.103

7 in total

12 in total

1. Broadly reactive nested reverse transcription-PCR using an internal RNA standard control for detection of noroviruses in stool samples.

Authors: Maria Cristina Medici; Monica Martinelli; Franco Maria Ruggeri; Laura Anna Abelli; Simona Bosco; Maria Cristina Arcangeletti; Federica Pinardi; Flora De Conto; Adriana Calderaro; Carlo Chezzi; Giuseppe Dettori
Journal: J Clin Microbiol Date: 2005-08 Impact factor: 5.948

2. Experience with an enzyme immunoassay for serotyping human group A rotaviruses.

Authors: L E Unicomb; B S Coulson; R F Bishop
Journal: J Clin Microbiol Date: 1989-03 Impact factor: 5.948

3. Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses.

Authors: B S Coulson; L E Unicomb; G A Pitson; R F Bishop
Journal: J Clin Microbiol Date: 1987-03 Impact factor: 5.948

4. Isolation, characterization, and host-cell-binding properties of a cytotoxin from Campylobacter jejuni.

Authors: S Mahajan; F G Rodgers
Journal: J Clin Microbiol Date: 1990-06 Impact factor: 5.948

5. A comparison of lyphogel, ammonium sulphate, and ultracentrifugation in the concentration of stool viruses for electron microscopy.

Authors: F G Rodgers; S Chapman; H Whitby
Journal: J Clin Pathol Date: 1981-02 Impact factor: 3.411

6. Heterologous protection against rotavirus-induced disease in gnotobiotic piglets.

Authors: R F Bishop; S R Tzipori; B S Coulson; L E Unicomb; M J Albert; G L Barnes
Journal: J Clin Microbiol Date: 1986-12 Impact factor: 5.948

7. Comparison between children treated at home and those requiring hospital admission for rotavirus and other enteric pathogens associated with acute diarrhea in Melbourne, Australia.

Authors: G A Pitson; K Grimwood; B S Coulson; F Oberklaid; A S Hewstone; I Jack; R F Bishop; G L Barnes
Journal: J Clin Microbiol Date: 1986-09 Impact factor: 5.948

8. Patients with enteric adenovirus gastroenteritis admitted to an Australian pediatric teaching hospital from 1981 to 1992.

Authors: K Grimwood; R Carzino; G L Barnes; R F Bishop
Journal: J Clin Microbiol Date: 1995-01 Impact factor: 5.948

Review 9. Diagnostic virology using electron microscopic techniques.

Authors: A M Field
Journal: Adv Virus Res Date: 1982 Impact factor: 9.937

10. Use of protein A in the serum-in-agar diffusion method in immune electron microscopy for detection of virus particles in cell culture.

Authors: S Furui
Journal: Microbiol Immunol Date: 1986 Impact factor: 1.955