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Literature DB >> 6995488

A quantitative study of the influence of fixation on immunoperoxidase staining of rectal mucosal plasma cells.

Abstract

A quantitative morphometric study of the immunoglobulin-containing plasma cells in rectal biopsies from nine patients with ulcerative colitis and three patients with the irritable colon syndrome is presented. The results show that fixation of the biopsy specimens with formol-sublimate, a mercuric fixative, resulted in better staining with the immunoperoxidase method and that, by comparison with formol saline, higher cell counts were always obtained.

Entities: Chemical Disease Species

Mesh：

Substances：
Fixatives

Year: 1980 PMID： 6995488 PMCID： PMC1146079 DOI： 10.1136/jcp.33.4.361

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

6 in total

1. The influence of fixation on immunoperoxidase staining of plasmacells in paraffin sections of intestinal biopsy specimens.

Authors: F T Bosman; J Lindeman; G Kuiper; A van der Wal; J Kreunig
Journal: Histochemistry Date: 1977-07-18

2. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody.

Authors: C R Taylor; J Burns
Journal: J Clin Pathol Date: 1974-01 Impact factor: 3.411

3. Immunohistochemical studies of the formation and epithelial transport of immunoglobulins in normal and diseased human intestinal mucosa.

Authors: P Brandtzaeg; K Baklien
Journal: Scand J Gastroenterol Suppl Date: 1976

4. Morphological methods in the study of the gut immune system in man.

Authors: J M Skinner; R Whitehead
Journal: J Clin Pathol Date: 1976-06 Impact factor: 3.411

5. Tissue eosinophils in ulcerative colitis.

Authors: C P Willoughby; J Piris; S C Truelove
Journal: Scand J Gastroenterol Date: 1979 Impact factor: 2.423

6. The plasma cells in inflammatory disease of the colon: a quantitative study.

Authors: J M Skinner; R Whitehead
Journal: J Clin Pathol Date: 1974-08 Impact factor: 3.411

6 in total

10 in total

1. Human intestinal mucosal mast cells: evaluation of fixation and staining techniques.

Authors: S Strobel; H R Miller; A Ferguson
Journal: J Clin Pathol Date: 1981-08 Impact factor: 3.411

2. Cellular immunolocalization of alpha-fetoprotein in rat liver.

Authors: J A Woods
Journal: Histochem J Date: 1983-10

3. Induction of maturation of human B-cell lymphomas in vitro. Morphologic changes in relation to immunoglobulin and DNA synthesis.

Authors: K Beiske; E Ruud; A Drack; P F Marton; T Godal
Journal: Am J Pathol Date: 1984-06 Impact factor: 4.307

4. Morphological and immunohistochemical findings in upper gastrointestinal biopsies of patients with Crohn's disease of the ileum and colon.

Authors: J P Van Spreeuwel; J Lindeman; A Van der Wal; I Weterman; J Kreuning; C Meijer
Journal: J Clin Pathol Date: 1982-09 Impact factor: 3.411

5. Influence of clearing agent on immunohistochemical staining of paraffin-embedded tissue.

Authors: J B Matthews
Journal: J Clin Pathol Date: 1981-01 Impact factor: 3.411

6. Plasma cell counts on human jejunal biopsy specimens examined by immunofluorescence and immunoperoxidase techniques: a comparative study.

Authors: D Kingston; J R Pearson; F J Penna
Journal: J Clin Pathol Date: 1981-04 Impact factor: 3.411

7. In vivo bromodeoxyuridine incorporation in normal mouse kidney: immunohistochemical detection and measurement of labelling indices.

Authors: H Robertson; J Wheeler; A R Morley
Journal: Histochem J Date: 1990-04