Literature DB >> 6993865

An enrichment method for heme-less mutants of Saccharomyces cerevisiae based on photodynamic properties of Zn-protoporphyrin.

D Grimal, R Labbe-Bois.   

Abstract

A new and widely applicable technique has been elaborated for enrichment of mutants of Saccharomyces cerevisiae deficient in porphyrin and heme synthesis. The method is based on selective photooxidative killing of the wild-type cells, sensitited by Zn-protoporphyrin synthesized and accumulated in wild-type cells under defined conditions. Using a single cycle of selection, heme-deficient mutants have been obtained with a frequency of 2-2.3%.

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Year:  1980        PMID： 6993865     DOI： 10.1007/bf00337883

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  16 in total

Review 1.  Porphyrins and related compounds as photodynamic sensitizers.

Authors:  J D Spikes
Journal:  Ann N Y Acad Sci       Date:  1975-04-15       Impact factor: 5.691

2.  Changes in the activities of the protoheme-synthesizing system during the growth of yeast under different conditions.

Authors:  R Labbe-Bois; C Volland
Journal:  Arch Biochem Biophys       Date:  1977-03       Impact factor: 4.013

3.  [Inhibition of the yeast respiratory system by Zn-protoporphyrin and effect of photolysis of this substance].

Authors:  F H Djavadi; M Moradi; F Etemad-Pour; L Djavadi-Ohaniance
Journal:  Biochimie       Date:  1977       Impact factor: 4.079

4.  Isolation of pleiotropic yeast mutants requiring ergosterol for growth.

Authors:  F Karst; F Lacroute
Journal:  Biochem Biophys Res Commun       Date:  1973-06-08       Impact factor: 3.575

5.  Phenotype of a temperature-sensitive, respiration-deficient (cyt) mutant of yeast.

Authors:  S Miyake; Y Iwamoto; M Nagao; T Sugimura; M Osumi
Journal:  J Bacteriol       Date:  1972-01       Impact factor: 3.490

6.  [Effect of Zn-protoporphyrin accumulation by yeast cells on synthesis and activity of their respiratory system].

Authors:  A Gilardi; L Djavadi-Ohaniance; P Labbe; P Chaix
Journal:  Biochim Biophys Acta       Date:  1971-06-15

7.  [New technic for determining absorption spectra at low temperature].

Authors:  P Labbe; P Chaix
Journal:  Bull Soc Chim Biol (Paris)       Date:  1969

8.  On the mode of cytotoxic action of photo-activated porphyrins.

Authors:  K Kohn; D Kessel
Journal:  Biochem Pharmacol       Date:  1979-08-15       Impact factor: 5.858

9.  Regulation of mitochondrial biogenesis: enzymatic changes in cytochrome-deficient yeast mutants requiring delta-aminolevulinic acid.

Authors:  R A Woods; H K Sanders; M Briquet; F Foury; B E Drysdale; J R Mattoon
Journal:  J Biol Chem       Date:  1975-12-10       Impact factor: 5.157

10.  Analysis of heme biosynthesis in catalase and cytochrome deficient yeast mutants.

Authors:  R Labbe-Bois; J Rytka; J Litwinska; T Bilinski
Journal:  Mol Gen Genet       Date:  1977-11-14
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  3 in total

1.  Isolation and characterization of a new mutant of Saccharomyces cerevisiae with altered synthesis of 5-aminolevulinic acid.

Authors:  E Carvajal; A D Panek; J R Mattoon
Journal:  J Bacteriol       Date:  1990-06       Impact factor: 3.490

2.  Genetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis.

Authors:  D Urban-Grimal; R Labbe-Bois
Journal:  Mol Gen Genet       Date:  1981

3.  405 nm and 450 nm Photoinactivation of Saccharomyces cerevisiae.

Authors:  K Hoenes; M Hess; P Vatter; B Spellerberg; M Hessling
Journal:  Eur J Microbiol Immunol (Bp)       Date:  2018-12-06
  3 in total

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