Literature DB >> 6993339

Pattern of chromosomal replication in synchronized lymphocytes. I. Evaluation and application of methotrexate block.

M Camargo, J Cervenka.   

Abstract

The BrdUrd-Hoechst 33258-Giemsa technique was employed to study patterns of chromosomal replication in human lymphocytes synchronized by Methotrexate (MTX). It is proposed that in the presence of MTX, a major portion of the cell population is blocked in an advanced stage of the S-phase and not in the G1/S border of the cell cycle. At this point, the replication of the chromosomal segments corresponding to the R-bands is terminated, and the replication of the G-bands and the inactive X-chromosomes is being initiated. The use of this method in the study of higher resolution patterns of chromosomal replications is proposed.

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Year:  1980        PMID： 6993339     DOI： 10.1007/bf00279048

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  32 in total

1.  Chromosome preparations of leukocytes cultured from human peripheral blood.

Authors:  P S MOORHEAD; P C NOWELL; W J MELLMAN; D M BATTIPS; D A HUNGERFORD
Journal:  Exp Cell Res       Date:  1960-09       Impact factor: 3.905

2.  High resolution of human chromosomes.

Authors:  J J Yunis
Journal:  Science       Date:  1976-03-26       Impact factor: 47.728

3.  [Staining of human chromosomes with acridine orange after treatment with 5 bromodeoxyuridine].

Authors:  B Dutrillaux; C Laurent; J Couturier; J Lejeune
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1973-06-13

4.  Replicon origins in Chinese hamsters cell DNA. I. Labeling procedure and preliminary observations.

Authors:  F Amaldi; F Carnevali; L Leoni; D Mariotti
Journal:  Exp Cell Res       Date:  1972-10       Impact factor: 3.905

5.  Distribution of DNA replicator sites in mammalian nuclei. II. Effects of prolonged inhibition of DNA synthesis.

Authors:  C H Ockey
Journal:  Exp Cell Res       Date:  1972-01       Impact factor: 3.905

6.  Sequence of DNA replication in 277 R- and Q-bands of human chromosomes using a BrdU treatment.

Authors:  B Dutrillaux; J Couturier; C L Richer; E Viegas-Péquignot
Journal:  Chromosoma       Date:  1976-10-12       Impact factor: 4.316

7.  The location of four human satellite DNAs on human chromosomes.

Authors:  J R Gosden; A R Mitchell; R A Buckland; R P Clayton; H J Evans
Journal:  Exp Cell Res       Date:  1975-04       Impact factor: 3.905

8.  DNA replication patterns of human chromosomes from fibroblasts and amniotic fluid cells revealed by a Giemsa staining technique.

Authors:  J T Epplen; J W Siebers; W Vogel
Journal:  Cytogenet Cell Genet       Date:  1975

9.  A high resolution study of the DNA replication patterns of chinese hamster chromosomes using sister chromatid differential staining technique.

Authors:  P E Crossen; S Pathak; F E Arrighi
Journal:  Chromosoma       Date:  1975-11-11       Impact factor: 4.316

10.  Fluorescence analysis of late DNA replication in human metaphase chromosomes.

Authors:  S A Latt
Journal:  Somatic Cell Genet       Date:  1975-07
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  25 in total

1.  Karyotype comparison and phylogenetic relationships of Pipistrellus-like bats (Vespertilionidae; Chiroptera; Mammalia).

Authors:  M Volleth; G Bronner; M C Göpfert; K G Heller; H S Yong
Journal:  Chromosome Res       Date:  2001       Impact factor: 5.239

2.  Analysis of DNA replication during S-phase by means of dynamic chromosome banding at high resolution.

Authors:  R Drouin; N Lemieux; C L Richer
Journal:  Chromosoma       Date:  1990-08       Impact factor: 4.316

3.  Replication asynchrony between homologs 15q11.2: cytogenetic evidence for genomic imprinting.

Authors:  Y Izumikawa; K Naritomi; K Hirayama
Journal:  Hum Genet       Date:  1991-05       Impact factor: 4.132

4.  The gene for the type 1 tumor necrosis factor receptor (TNF-R1) is localized on band 12p13.

Authors:  J Derré; O Kemper; D Cherif; Y Nophar; R Berger; D Wallach
Journal:  Hum Genet       Date:  1991-06       Impact factor: 4.132

5.  Characterization of chromosome replication during S-phase with bromodeoxyuridine labelling in Chinese hamster ovary and HeLa cells.

Authors:  W Vogel; S Boldin; A Reisacher; G Speit
Journal:  Chromosoma       Date:  1985       Impact factor: 4.316

6.  Simultaneous localization of cosmids and chromosome R-banding by fluorescence microscopy: application to regional mapping of human chromosome 11.

Authors:  D Cherif; C Julier; O Delattre; J Derré; G M Lathrop; R Berger
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

Review 7.  Evolution of the Simiiformes and the phylogeny of human chromosomes.

Authors:  I C Clemente; M Ponsà; M García; J Egozcue
Journal:  Hum Genet       Date:  1990-05       Impact factor: 4.132

8.  Centromeric association and non-random distribution of centromeres in human tumour cells.

Authors:  T Haaf; M Schmid
Journal:  Hum Genet       Date:  1989-01       Impact factor: 4.132

9.  High resolution R banding in amniotic fluid cells using the BrdU-Hoechst 33258-Giemsa (RBG) technique.

Authors:  S W Cheung; J P Crane; A C Burgess
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

10.  Replication variants of the human inactive X chromosome. I. Variability within lymphocytes of single individuals.

Authors:  M Schmidt; W M Stolzmann; L I Baranovskaya
Journal:  Chromosoma       Date:  1982       Impact factor: 4.316
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