Literature DB >> 6993050

Enzymatic activities on purine pyrimidine metabolism in nine mycoplasma species contaminating cell cultures.

M Hamet, C Bonissol, P Cartier.   

Abstract

The study of mycoplasma enzymes involved in nucleotide degradation and base salvage pathways allowed determination of enzyme profiles for some species. The activity of adenosine phosphorylase seems to be the biochemical test of choice to detect mycoplasma contamination in cell cultures. All mycoplasmas tested had a very high level of activity compared to very low activity in human cell cultures, rendering the technic sensitive even at a low degree of contamination.

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Year:  1980        PMID: 6993050     DOI: 10.1016/0009-8981(80)90225-9

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN: 0009-8981            Impact factor:   3.786


  11 in total

1.  A method for enrichment of hybrid somatic cells: complementation studies in certain lysosomal enzymopathies.

Authors:  P V Nelson; W F Carey
Journal:  J Inherit Metab Dis       Date:  1985       Impact factor: 4.982

Review 2.  Cytogenetic effects of mycoplasmal infection of cell cultures: a review.

Authors:  G J McGarrity; V Vanaman; J Sarama
Journal:  In Vitro       Date:  1984-01

3.  De novo purine synthesis, purine salvage, and DNA synthesis in normal and Lesch-Nyhan fibroblasts infected with Mycoplasma pneumoniae.

Authors:  S Upchurch; M G Gabridge
Journal:  Infect Immun       Date:  1983-01       Impact factor: 3.441

4.  Synthesis of deoxyribomononucleotides in Mollicutes: dependence on deoxyribose-1-phosphate and PPi.

Authors:  M C McElwain; J D Pollack
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

5.  Uracil phosphoribosyltransferase from Acholeplasma laidlawii: partial purification and kinetic properties.

Authors:  R S McIvor; R M Wohlhueter; P G Plagemann
Journal:  J Bacteriol       Date:  1983-10       Impact factor: 3.490

6.  Uridine phosphorylase from Acholeplasma laidlawii: purification and kinetic properties.

Authors:  R S McIvor; R M Wohlhueter; P G Plagemann
Journal:  J Bacteriol       Date:  1983-10       Impact factor: 3.490

7.  Induction of adenine salvage in mouse cell lines deficient in adenine phosphoribosyltransferase.

Authors:  M S Turker; G M Martin
Journal:  Mol Cell Biol       Date:  1985-10       Impact factor: 4.272

8.  Cultivation and partial characterization of spiroplasmas in cell cultures.

Authors:  T Steiner; G J McGarrity; D M Phillips
Journal:  Infect Immun       Date:  1982-01       Impact factor: 3.441

9.  Purine metabolism in Acholeplasma laidlawii B: novel PPi-dependent nucleoside kinase activity.

Authors:  V V Tryon; D Pollack
Journal:  J Bacteriol       Date:  1984-07       Impact factor: 3.490

Review 10.  The metabolic pathways of Acholeplasma and Mycoplasma: an overview.

Authors:  J D Pollack; V V Tryon; K D Beaman
Journal:  Yale J Biol Med       Date:  1983 Sep-Dec
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