Literature DB >> 6992770

Bioautographic visualization of dihydrofolate reductase in enzyme overproducing BHK mutants.

S L Naylor, J K Townsend, R J Klebe.   

Abstract

A bioautographic procedure has been developed for the visualization of the isozymes of dihydrofolate reductase (DHFR, E.C. 1.5.1.3). In addition to detecting electrophoretically separated enzymes, bioautography was utilized to visualize DHFR after isoelectric focusing on polyacrylamide gels. Both zone electrophoresis and isoelectric focusing were used to compare wildtype BHK cells to mutants which overproduce dihydrofolate reductase. In agreement with other physical data, the BHK-A5 overproduction mutant appears to produce more dihydrofolate reductase of the same electrophoretic mobility and isoelectric point as wild type cells.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 6992770     DOI： 10.1007/bf00504369

Source DB:  PubMed          Journal:  Biochem Genet        ISSN： 0006-2928            Impact factor:   1.890


  16 in total

1.  Relation of folic acid reductase to amethopterin resistance in cultured mammalian cells.

Authors:  M T HAKALA; S F ZAKRZEWSKI; C A NICHOL
Journal:  J Biol Chem       Date:  1961-03       Impact factor: 5.157

2.  Altered regulation of the rate of synthesis of dihydrofolate reductase in methotrexate-resistant hamster cells.

Authors:  U J Hänggi; J W Littlefield
Journal:  J Biol Chem       Date:  1976-05-25       Impact factor: 5.157

3.  Elevated dihydrofolate reductase messenger RNA levels in methotrexate-resistant BHK cells.

Authors:  S E Chang; J W Littlefield
Journal:  Cell       Date:  1976-03       Impact factor: 41.582

4.  Two different species of dihydrofolate reductase in mammalian cells differentially resistant to amethopterin and methasquin.

Authors:  A M Albrecht; J L Biedler; D J Hutchison
Journal:  Cancer Res       Date:  1972-07       Impact factor: 12.701

5.  Purification, properties, and synthesis of dihydrofolate reductase from wild type and methotrexate-resistant hamster cells.

Authors:  H Nakamura; J W Littlefield
Journal:  J Biol Chem       Date:  1972-01-10       Impact factor: 5.157

Review 6.  Folic acid metabolism.

Authors:  E L Stokstad; J Koch
Journal:  Physiol Rev       Date:  1967-01       Impact factor: 37.312

7.  Sites of action of amethopterin: intrinsic and acquired drug resistance.

Authors:  K R Harrap; B T Hill; M E Furness; L I Hart
Journal:  Ann N Y Acad Sci       Date:  1971-11-30       Impact factor: 5.691

8.  Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

Authors:  F W Alt; R E Kellems; J R Bertino; R T Schimke
Journal:  J Biol Chem       Date:  1978-03-10       Impact factor: 5.157

9.  Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cells.

Authors:  W F Flintoff; S V Davidson; L Siminovitch
Journal:  Somatic Cell Genet       Date:  1976-05

10.  Detective transport of amethopterin (methotrexate) as a mechanism of resistance to the antimetabolite in L5178Y leukemic cells.

Authors:  G A FISCHER
Journal:  Biochem Pharmacol       Date:  1962-12       Impact factor: 5.858
View more
  1 in total

1.  Two-dimensional spectroscopy of electrophoretic gels.

Authors:  R J Klebe; M G Mancuso; C R Brown; L Teng
Journal:  Biochem Genet       Date:  1981-08       Impact factor: 1.890
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.