Literature DB >> 6990408

Primary structure of elongation factor Tu from Escherichia coli.

K Arai, B F Clark, L Duffy, M D Jones, Y Kaziro, R A Laursen, J L'Italien, D L Miller, S Nagarkatti, S Nakamura, K M Nielsen, T E Petersen, K Takahashi, M Wade.   

Abstract

The amino acid sequence of elongation factor Tu (EF-Tu) from Escherichia coli has been determined. EF-Tu is a single-chain polypeptide composed of 393 amino acids (Mr 43,225 for the species bearing COOH-terminal serine). The NH2-terminal serine is acetylated, and lysine-56 is partially methylated. The sites of facile tryptic cleavage are at arginines 44 and 58 and at lysine-263. The cysteinyl residues associated with aminoacyl-tRNA and guanosine nucleotide binding activities are residues 81 and 137, respectively. The COOH-terminal amino acid is heterogenous in that analyses of the COOH-terminal peptides isolated from different EF-Tu preparations gave position 393 as glycine and serine in ratios (Gly/Ser) ranging from about 0.7 to 3.

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Year:  1980        PMID： 6990408      PMCID： PMC348487          DOI： 10.1073/pnas.77.3.1326

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  45 in total

1.  Location of the site of methylation in elongation factor Tu.

Authors:  J J L'Italien; R A Laursen
Journal:  FEBS Lett       Date:  1979-11-15       Impact factor: 4.124

2.  Studies on polypeptide elongation factors from Escherichia coli. II. Purification of factors Tu-guanosine diphosphate, Ts, and Tu-Ts, and crystallization of Tu-guanosine diphosphate and Tu-Ts.

Authors:  K I Arai; M Kawakita; Y Kaziro
Journal:  J Biol Chem       Date:  1972-11-10       Impact factor: 5.157

3.  Studies on the polypeptide elongation factors from E. coli. 3. Molecular characteristics of EF-Tu-guanosine diphosphate, EP-Ts, and EF-Tu-Ts complex.

Authors:  K Arai; M Kawakita; Y Kaziro; T Kondo; N Ui
Journal:  J Biochem       Date:  1973-05       Impact factor: 3.387

4.  Amino-terminal sequence analysis of proteins purified on a nanomole scale by gel electrophoresis.

Authors:  A M Weiner; T Platt; K Weber
Journal:  J Biol Chem       Date:  1972-05-25       Impact factor: 5.157

5.  Studies on the purification and properties of factor Tu from E. coli.

Authors:  D L Miller; H Weissbach
Journal:  Arch Biochem Biophys       Date:  1970-11       Impact factor: 4.013

6.  Solid-phase Edman degradation. An automatic peptide sequencer.

Authors:  R A Laursen
Journal:  Eur J Biochem       Date:  1971-05-11

7.  Involvement of histidine residues in the substrate binding of elongation factor Tu from Thermus thermophilus: proton nuclear magnetic resonance and photooxidation study.

Authors:  A Nakano; T Miyazawa; S Nakamura; Y Kaziro
Journal:  Arch Biochem Biophys       Date:  1979-08       Impact factor: 4.013

8.  Determination of the amino acid sequence of the monkey, sheep, and dog proinsulin C-peptides by a semi-micro Edman degradation procedure.

Authors:  J D Peterson; S Nehrlich; P E Oyer; D F Steiner
Journal:  J Biol Chem       Date:  1972-08-10       Impact factor: 5.157

9.  The reactions of the sulfhydryl groups on the elongation factors Tu and Ts.

Authors:  D L Miller; J Hachmann; H Weissbach
Journal:  Arch Biochem Biophys       Date:  1971-05       Impact factor: 4.013

Review 10.  Protein biosynthesis.

Authors:  J Lucas-Lenard
Journal:  Annu Rev Biochem       Date:  1971       Impact factor: 23.643
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  57 in total

1.  Nucleotide sequence of a DNA region comprising the gene for elongation factor 1 alpha (EF-1 alpha) from the ultrathermophilic archaeote Pyrococcus woesei: phylogenetic implications.

Authors:  R Creti; F Citarella; O Tiboni; A Sanangelantoni; P Palm; P Cammarano
Journal:  J Mol Evol       Date:  1991-10       Impact factor: 2.395

2.  Specific peptide-activated proteolytic cleavage of Escherichia coli elongation factor Tu.

Authors:  T Georgiou; Y N Yu; S Ekunwe; M J Buttner; A Zuurmond; B Kraal; C Kleanthous; L Snyder
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-17       Impact factor: 11.205

3.  Molecular cloning and sequence determination of cDNAs for alpha subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain.

Authors:  H Itoh; T Kozasa; S Nagata; S Nakamura; T Katada; M Ui; S Iwai; E Ohtsuka; H Kawasaki; K Suzuki
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

4.  GTP-binding domain: three consensus sequence elements with distinct spacing.

Authors:  T E Dever; M J Glynias; W C Merrick
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

5.  Isolation, characterization and mRNA expression of four cDNAs encoding translation elongation factor 1A from rice (Oryza sativa L.).

Authors:  S Kidou; S Ejiri
Journal:  Plant Mol Biol       Date:  1998-01       Impact factor: 4.076

6.  Precursor for elongation factor Tu from Escherichia coli.

Authors:  E R Lifson; L Lindahl; J M Zengel
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490

Review 7.  Properties and regulation of the GTPase activities of elongation factors Tu and G, and of initiation factor 2.

Authors:  A Parmeggiani; G Sander
Journal:  Mol Cell Biochem       Date:  1981-03-27       Impact factor: 3.396

8.  Translation elongation factor Tu cleaved by a phage-exclusion system.

Authors:  Y T Yu; L Snyder
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-18       Impact factor: 11.205

9.  Estimation of deuteration levels in whole cells and cellular proteins by 1H n.m.r. spectroscopy and neutron scattering.

Authors:  S J Perkins
Journal:  Biochem J       Date:  1981-10-01       Impact factor: 3.857

10.  Shotgun proteomics of the haloarchaeon Haloferax volcanii.

Authors:  P Aaron Kirkland; Matthew A Humbard; Charles J Daniels; Julie A Maupin-Furlow
Journal:  J Proteome Res       Date:  2008-09-25       Impact factor: 4.466
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