Literature DB >> 6989522

Improved reaction buffers for solid-phase enzyme immunoassay without interference by serum factors.

K Kato, Y Umeda, F Suzuki, A Kosaka.   

Abstract

A gelatin moderately digested by a protease, was as effective as original gelatin to remove the interference by serum factors with a sandwich immunoassay for insulin. Digestion of gelatin resulted in decrease in the viscosity, and the buffer containing the digested gelatin did not show gelation at 4 degrees C. Therefore the buffer involving the digested gelatin might be widely useful for the immunoassay without interference by serum factors.

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Year:  1980        PMID: 6989522     DOI: 10.1016/0009-8981(80)90043-1

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN: 0009-8981            Impact factor:   3.786


  1 in total

1.  Tyrosine hydroxylase indicates cell differentiation of catecholamine biosynthesis in neuroendocrine tumors.

Authors:  K Iwase; A Nagasaka; I Nagatsu; K Kiuchi; T Nagatsu; H Funahashi; T Tsujimura; A Inagaki; A Nakai; T Kishikawa
Journal:  J Endocrinol Invest       Date:  1994-04       Impact factor: 4.256

  1 in total

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