Literature DB >> 6985356

A novel mRNA and a low molecular weight polypeptide encoded in the transforming region of adenovirus DNA.

M G Katze, H Persson, L Philipson.   

Abstract

Immunoprecipitation was used to identify adenovirus type 2 (ad2) tumor antigens synthesized in vivo. The antisera, prepared from tumor-bearing animals, reacted with a wide spectrum of ad2 early proteins including a 11 000-dalton (11 K) polypeptide. The gene for this polypeptide was mapped to the transforming region of the viral genome by hybridization selection followed by in vitro translation and immunoprecipitation. Hybrid arrest translation revealed that the 11 K RNA was transcribed from the leftward reading strand (1-strand) in contrast to other mRNAs from this region. Sucrose gradient analysis of the selected 11 K mRNA revealed that the size of the mRNA was 20S corresponding to approximately 2000 nucleotides. Novel 1-strand transcripts of this length from the transforming region were identified by S1 endonuclease analysis. Taken together, these results suggest that both strands of the transforming region of ad2 DNA are actively transcribed into functional mRNA early after viral infection.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 6985356      PMCID： PMC553110          DOI： 10.1002/j.1460-2075.1982.tb01248.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  36 in total

1.  Control of adenovirus early gene expression: posttranscriptional control mediated by both viral and cellular gene products.

Authors:  M G Katze; H Persson; L Philipson
Journal:  Mol Cell Biol       Date:  1981-09       Impact factor: 4.272

2.  Immunological identification of two adenovirus 2-induced early proteins possibly involved in cell transformation.

Authors:  Z Gilead; Y H Jeng; W S Wold; K Sugawara; H M Rho; M L Harter; M Green
Journal:  Nature       Date:  1976-11-18       Impact factor: 49.962

3.  Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and simian virus 40.

Authors:  F L Graham; P J Abrahams; C Mulder; H L Heijneker; S O Warnaar; F A De Vries; W Fiers; A J Van Der Eb
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1975

4.  Hybridization maps of early and late messenger RNA sequences on the adenovirus type 2 genome.

Authors:  U Pettersson; C Tibbetts; L Philipson
Journal:  J Mol Biol       Date:  1976-03-15       Impact factor: 5.469

5.  Location and identification of the genes for adenovirus type 2 early polypeptides.

Authors:  J B Lewis; J F Atkins; P R Baum; R Solem; R F Gesteland; C W Anderson
Journal:  Cell       Date:  1976-01       Impact factor: 41.582

6.  The isolation and identification of the adenovirus group C tumor antigens.

Authors:  A Levinson; A J Levine
Journal:  Virology       Date:  1977-01       Impact factor: 3.616

7.  Purification and characterization of the phosphorylated DNA-binding protein from adenovirus-type-2-infected cells.

Authors:  T Linné; H Jörnvall; L Philipson
Journal:  Eur J Biochem       Date:  1977-06-15

8.  Effect of protein synthesis inhibitors on viral mRNA's synthesized early in adenovirus type 2 infection.

Authors:  F Eggerding; H J Raskas
Journal:  J Virol       Date:  1978-01       Impact factor: 5.103

9.  Relationship of mRNA from productively infected cells to the complementary strands of adenovirus type 2 DNA.

Authors:  C Tibbetts; U Pettersson; K Johansson; L Philpson
Journal:  J Virol       Date:  1974-02       Impact factor: 5.103

10.  Structure of the adenovirus 2 early mRNAs.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1978-07       Impact factor: 41.582
View more
  9 in total

1.  The E1B 19-kilodalton protein is not essential for transformation of rodent cells in vitro by adenovirus type 5.

Authors:  G C Telling; J Williams
Journal:  J Virol       Date:  1993-03       Impact factor: 5.103

2.  The interplay between host and viral genes in adenovirus gene expression.

Authors:  L Philipson
Journal:  Klin Wochenschr       Date:  1984-05-15

3.  Control of adenovirus gene expression: cellular gene products restrict expression of adenovirus host range mutants in nonpermissive cells.

Authors:  M G Katze; H Persson; B M Johansson; L Philipson
Journal:  J Virol       Date:  1983-04       Impact factor: 5.103

4.  Requirement for either early region 1a or early region 1b adenovirus gene products in the helper effect for adeno-associated virus.

Authors:  W D Richardson; H Westphal
Journal:  J Virol       Date:  1984-08       Impact factor: 5.103

5.  Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli.

Authors:  K R Spindler; D S Rosser; A J Berk
Journal:  J Virol       Date:  1984-01       Impact factor: 5.103

6.  Deletion of the gene encoding the adenovirus 5 early region 1b 21,000-molecular-weight polypeptide leads to degradation of viral and host cell DNA.

Authors:  S Pilder; J Logan; T Shenk
Journal:  J Virol       Date:  1984-11       Impact factor: 5.103

7.  The adenovirus E1B-55K transforming polypeptide modulates transport or cytoplasmic stabilization of viral and host cell mRNAs.

Authors:  S Pilder; M Moore; J Logan; T Shenk
Journal:  Mol Cell Biol       Date:  1986-02       Impact factor: 4.272

8.  Activation of adenovirus 5 E1A transcription by region E1B in transformed primary rat cells.

Authors:  A G Jochemsen; L T Peltenburg; M F te Pas; C M de Wit; J L Bos; A J van der Eb
Journal:  EMBO J       Date:  1987-11       Impact factor: 11.598

9.  Tupaia (tree shrew) adenovirus DNA: sequence of the left-hand fragment corresponding to the transforming early region of human adenoviruses.

Authors:  U Brinckmann; G Darai; R M Flügel
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.