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Literature DB >> 698344

Fluorescein excitation and emission polarization spectra in living cells: changes during the cell cycle.

Abstract

Changes in the fluorescein fluorescence emission and excitation polarization spectra in synchronized cultured S3 fibroblasts at G1, mid-S, and mitosis, as well as in human lymphocytes before and after stimulation with mitogens, were studied. In contrast to those measured in aqueous solutions the emission and excitation polarization spectra in living cells exhibit a wavelength dependence characteristic for the state of the cell cycle. Changes in the temperature and in the amount of intracellular water result in quantitative wavelength-independent changes in the polarization spectra. Possible mechanisms for the qualitative wavelength-dependent changes in the fluorescein emission and excitation polarization spectra during the cell cycle are discussed.

Entities: Chemical Species

Mesh：

Substances：
Fluoresceins
Mitogens

Year: 1978 PMID： 698344 PMCID： PMC1473529 DOI： 10.1016/S0006-3495(78)85458-7

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

13 in total

1. FLUORESCENCE POLARIZATION: MEASUREMENT WITH ULTRAVIOLET-POLARIZING FILTERS IN A SPECTROPHOTOFLUOROMETER.

Authors: R F CHEN; R L BOWMAN
Journal: Science Date: 1965-02-12 Impact factor: 47.728

2. Polarization of the fluorescence of macromolecules. I. Theory and experimental method.

Authors: G WEBER
Journal: Biochem J Date: 1952-05 Impact factor: 3.857

Review 3. Application of the phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) in the diagnosis of malignant disorders: a review.

Authors: L Cercek; B Cercek
Journal: Eur J Cancer Date: 1977-09 Impact factor: 9.162

4. Relationship between changes in the structuredness of cytoplasm and rate constants for the hydrolysis of FDA in Saccharomyces cerevisiae.

Authors: L Cercek; B Cercek
Journal: Biophysik Date: 1973

5. Cytoplasmic solvent structure of single barnacle muscle cells studied by electron spin resonance.

Authors: F Sachs; R Latorre
Journal: Biophys J Date: 1974-04 Impact factor: 4.033

6. Rapid preparation of lymphocytes for tissue-typing.

Authors: R Harris; E O Ukaejiofo
Journal: Lancet Date: 1969-08-09 Impact factor: 79.321

7. Structuredness of the cytoplasmic matrix and Michaelis-Menten constants for the hydrolysis of FDA during the cell cycle in Chinese hamster ovary cells.

Authors: L Cercek; B Cercek; C H Ockey
Journal: Biophysik Date: 1973

8. Induction of PHA response in mouse bone marrow cells by thymic extracts as studied by changes in the structuredness of cytoplasmic matrix.

Authors: L Cercek; P Milenkovic; B Cercek; L G Lajtha
Journal: Immunology Date: 1975-11 Impact factor: 7.397

Fluorescein excitation and emission polarization spectra in living cells: changes during the cell cycle.

1. FLUORESCENCE POLARIZATION: MEASUREMENT WITH ULTRAVIOLET-POLARIZING FILTERS IN A SPECTROPHOTOFLUOROMETER.

2. Polarization of the fluorescence of macromolecules. I. Theory and experimental method.

Review 3. Application of the phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) in the diagnosis of malignant disorders: a review.

4. Relationship between changes in the structuredness of cytoplasm and rate constants for the hydrolysis of FDA in Saccharomyces cerevisiae.

5. Cytoplasmic solvent structure of single barnacle muscle cells studied by electron spin resonance.

6. Rapid preparation of lymphocytes for tissue-typing.

7. Structuredness of the cytoplasmic matrix and Michaelis-Menten constants for the hydrolysis of FDA during the cell cycle in Chinese hamster ovary cells.

8. Induction of PHA response in mouse bone marrow cells by thymic extracts as studied by changes in the structuredness of cytoplasmic matrix.

9. Flow cytometric measurement of the polarization of fluorescence from intracellular fluorescein in mammalian cells.

10. Inhibitors of haemopoietic cell proliferation? Specificity of action within the haemopoietic system.

1. Comments on the fluorescein excitation and emission polarization spectra in living cells.

2. Involvement of mitochondria in changes in fluorescein excitation and emission polarization spectra in living cells.

3. Epi-illumination optical design for fluorescence polarization measurements in flow systems.

4. Change of intracellular fluidity during keratinocyte differentiation measured by fluorescence polarization.

5. Comments on "the SCM test for cancer. An evaluation in terms of lymphocytes from healthy donors and cancer patients".

6. Effect of specific growth inhibitors on fluorescein fluorescence polarization spectra in haemopoietic cells.

7. The cellular basis for biocide-induced fluorescein hyperfluorescence in mammalian cell culture.

8. The SCM test for cancer. an evaluation in terms of lymphocytes from healthy donors and cancer patients.