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Literature DB >> 6981412

The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices.

M D Scawen, J Darbyshire, M J Harvey, T Atkinson.

Abstract

3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two sequential affinity-chromatography steps on two triazine dye-Sepharose matrices. Recoveries of both enzymes were in excess of 60%. Malate dehydrogenase could also be purified by a combination of triazine dye affinity chromatography and gel filtration on Ultrogel AcA-44, but this offered no significant advantage over the purely affinity procedure.

Entities: Chemical Gene

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Year: 1982 PMID： 6981412 PMCID： PMC1158286 DOI： 10.1042/bj2030699

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

17 in total

1. Enzymic determination of D(-)-beta-hydroxybutyric acid and acetoacetic acid in blood.

Authors: D H WILLIAMSON; J MELLANBY; H A KREBS
Journal: Biochem J Date: 1962-01 Impact factor: 3.857

2. Enzyme purification by substrate elution chromatography from procion dye-polysaccharide matrices.

Authors: J K Baird; R F Sherwood; R J Carr; A Atkinson
Journal: FEBS Lett Date: 1976-11 Impact factor: 4.124

3. Purification and properties of beta-hydroxybutyrate dehydrogenase from Mycobacterium phlei ATCC354.

Authors: K R Dhariwal; T A Venkitasubramanian
Journal: J Gen Microbiol Date: 1978-01

4. Tartaric acid metabolism. IV. Crystalline L-malic dehydrogenase from Pseudomonas acidovorans.

Authors: L D Kohn; W B Jakoby
Journal: J Biol Chem Date: 1968-05-25 Impact factor: 5.157

5. Malate dehydrogenases. II. Purification and properties of Bacillus subtilis, Bacillus stearothermophilus, and Escherichia coli malate dehydrogenases.

Authors: W H Murphey; C Barnaby; F J Lin; N O Kaplan
Journal: J Biol Chem Date: 1967-04-10 Impact factor: 5.157

4. Tandem dye-ligand chromatography and biospecific elution applied to the purification of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides.

Authors: Y Hey; P D Dean
Journal: Biochem J Date: 1983-02-01 Impact factor: 3.857

5. Purification and characterization of an (S)-3-hydroxycarboxylate oxidoreductase from Clostridium tyrobutyricum.

Authors: M Bayer; H Günther; H Simon
Journal: Appl Microbiol Biotechnol Date: 1994-10 Impact factor: 4.813

5 in total

The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices.

1. Enzymic determination of D(-)-beta-hydroxybutyric acid and acetoacetic acid in blood.

2. Enzyme purification by substrate elution chromatography from procion dye-polysaccharide matrices.

3. Purification and properties of beta-hydroxybutyrate dehydrogenase from Mycobacterium phlei ATCC354.

4. Tartaric acid metabolism. IV. Crystalline L-malic dehydrogenase from Pseudomonas acidovorans.

5. Malate dehydrogenases. II. Purification and properties of Bacillus subtilis, Bacillus stearothermophilus, and Escherichia coli malate dehydrogenases.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Purification and properties of malate dehydrogenase from Pseudomonas testosteroni.

8. Malate dehydrogenases. I. A survey of molecular size measured by gel filtration.

9. Preparation of gram quantities of a purified R-factor-mediated penicillinase from Escherichia coli strain W3310.

10. Simple efficient methods for the isolation of malate dehydrogenase from thermophilic and mesophilic bacteria.

1. The purification and characterization of glucokinase from the thermophile Bacillus stearothermophilus.

2. Malate dehydrogenase in phototrophic purple bacteria: purification, molecular weight, and quaternary structure.

3. Development of monoclonal antibodies to parathyroid hormone-induced resorptive factors from osteoblast-like cells.

4. Tandem dye-ligand chromatography and biospecific elution applied to the purification of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides.

5. Purification and characterization of an (S)-3-hydroxycarboxylate oxidoreductase from Clostridium tyrobutyricum.