Literature DB >> 6979540

A high yield purification procedure for alpha and beta chains of HLA-DR antigens.

L E Walker, R A Reisfeld.   

Abstract

A procedure is described for purifying to homogeneity the alpha and beta chains of HLA-DR antigen from the homozygous human lymphoid cell line LG-2. The procedure involves a combination of lectin affinity as well as classical and high pressure liquid chromatography. From 100 g of packed cells, 8 mg (235 nmol) of alpha chain and 7 mg (205 nmol) of beta chain were isolated.

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Year:  1982        PMID: 6979540

Source DB:  PubMed          Journal:  J Biol Chem        ISSN: 0021-9258            Impact factor:   5.157


  1 in total

1.  Sheep lymphocyte antigens (OLA). II. Major histocompatibility complex class II molecules.

Authors:  N K Puri; C R Mackay; M R Brandon
Journal:  Immunology       Date:  1985-12       Impact factor: 7.397

  1 in total

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