Literature DB >> 6978684

Pregnancy specific beta 1-glycoprotein (SP1) conversion of SP1 beta to SP1 alpha by "acidification": an artefact.

B Teisner, J G Grudzinskas.   

Abstract

Late pregnancy serum and plasma were examined in rocket immunoelectrophoresis (RIE) and crossed immunoelectrophoresis (XIE) before and after "acid treatment" (pH 2.5, 10 min). The analysis revealed changes in the precipitation morphology in the majority of circulating proteins extending from the albumin to the gamma-region. In RIE, the precipitation pattern of SP1 was altered, this change being independent of polyethylene glycol. In addition, XIE analysis of SP1 revealed a change in the precipitation morphology with no change in electrophoretic mobility. The results can be explained by non-specific aggregation and denaturation due to "acid treatment", and are therefore unlikely to be of any biological or immunochemical importance.

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Year:  1982        PMID： 6978684     DOI： 10.1007/BF02111666

Source DB:  PubMed          Journal:  Arch Gynecol        ISSN： 0170-9925


  10 in total

1.  Molecular heterogeneity of pregnancy specific beta 1 glycoprotein: the effect on measurement by radioimmunoassay and electroimmunoassay.

Authors:  B Teisner; J G Grudzinskas; P Hindersson; A T Al-Ani; J G Westergaard; T Chard
Journal:  J Immunol Methods       Date:  1979       Impact factor: 2.303

2.  The ratio between molecular variants of pregnancy-specific beta 1 glycoprotein (SP1) in the peripheral and retroplacental circulation during later pregnancy.

Authors:  J G Grudzinskas; B Teisner; T Chard
Journal:  Placenta       Date:  1980 Jul-Sep       Impact factor: 3.481

3.  Separation of two pregnancy-associated proteins with SP1 determinants and the conversion of SP1 beta to SP1 alpha.

Authors:  A G Ahmed; A Klopper
Journal:  Arch Gynecol       Date:  1980

4.  Specific assay for pregnancy-specific-beta 1-glycoprotein (Sp1-beta). Preparation of antiserum specific for SP1-beta by absorption with a crossreacting high molecular weight serum protein.

Authors:  P Hindersson; J Folkersen; J G Westergaard; B Teisner; S E Svehag
Journal:  J Reprod Immunol       Date:  1979-10       Impact factor: 4.054

5.  On molecular heterogeneity of pregnancy specific beta 1-glycoprotein.

Authors:  B Teisner; J G Grudzinskas
Journal:  Aust N Z J Obstet Gynaecol       Date:  1981-02       Impact factor: 2.100

6.  Immunochemical and biological studies on two molecular variants of pregnancy specific beta 1 glycoprotein (SP1) SP1 alpha and SP1 beta.

Authors:  J G Grudzinskas; B Teisner; A T Al-Ani; J Folkersen; J G Westergaard; T Chard
Journal:  Clin Chim Acta       Date:  1980-09-08       Impact factor: 3.786

7.  Investigations into the molecular heterogeneity of pregnancy-specific beta 1-glycoprotein (SP1).

Authors:  P Hindersson; B Teisner; J Folkersen; J G Grudzinskas; J G Westergaard
Journal:  Placenta       Date:  1981 Jul-Sep       Impact factor: 3.481

8.  Characterization of two pregnancy associated serum proteins with pregnancy specific beta 1-glycoprotein (PS-beta-G)-determinants.

Authors:  J G Westergaard; B Teisner; J Folkersen; P Hindersson; P Schultz-Larsen; S E Svehag
Journal:  Scand J Clin Lab Invest       Date:  1979-06       Impact factor: 1.713

9.  Quantification of the pregnancy-specific beta1-glycoprotein (SP1) by immunoprecipitation techniques: the influence of a cross-reacting high molecular weight alpha2-protein.

Authors:  B Teisner; J Folkersen; P Hindersson; J C Jensenius; J G Westergaard
Journal:  Scand J Immunol       Date:  1979       Impact factor: 3.487

10.  Two pregnancy-associated serum proteins with pregnancy-specific beta1-glycoprotein determinants.

Authors:  B Teisner; J G Westergaard; J Folkersen; S Husby; S E Svehag
Journal:  Am J Obstet Gynecol       Date:  1978-06-01       Impact factor: 8.661
  10 in total

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