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Literature DB >> 697748

A method for the analysis of protein turnover characteristics. Indirect estimation of rates of protein degradation.

Abstract

A method for the calculation of rate constants of degradation of a specific protein during a period of change in protein amount is described. The calculation uses measurements of rates of synthesis and amount of the protein, together with one estimate of the protein half-life. The method is demonstrated by using data from measurements made on the accumulation of fatty acid synthetase in hormonally treated explants of mammary gland from mid-pregnant rabbits, and data from other authors. These calculations demonstrate a regulated programme of co-ordinated changes in rates of synthesis and degradation during a period of change in protein mass. The role of such changes in protein degradation during protein accumulation is discussed.

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Year: 1978 PMID： 697748 PMCID： PMC1185895 DOI： 10.1042/bj1740153

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

22 in total

1. Effects of estradiol and nicotinamide adenine dinucleotide phosphate on rate of degradation of uterine glucose-6-phosphate dehydrogenase.

Authors: E R Smith; K L Barker
Journal: J Biol Chem Date: 1977-06-10 Impact factor: 5.157

A method for the analysis of protein turnover characteristics. Indirect estimation of rates of protein degradation.

1. Effects of estradiol and nicotinamide adenine dinucleotide phosphate on rate of degradation of uterine glucose-6-phosphate dehydrogenase.

2. The kinetics of catalase synthesis and destruction in vivo.

3. Kinetics of binding of cytochrome c oxidase from rat liver to an immunoadsorbent [proceedings].

4. Molecular weight and subunit size of fatty acid synthetase from rabbit mammary gland.

5. Faster synthesis and slower degradation of liver protein during developmental growth.

Review 6. Control of enzyme levels in animal tissues.

7. Myosin synthesis and degradation during development of cardiac hypertrophy in the rabbit.

8. Characterization of radioactive immunochemically precipitated protein antigens using SDS-polyacrylamide gels.

9. The role of enzyme degradation in enzyme turnover during tissue differentiation.

10. Effect of dietary linoleate on synthesis and degradation of fatty acid synthetase from rat liver.

1. Lipogenic enzymes in rat maternal adipose tissue in the perinatal period.

2. Protein degradation during terminal cytodifferentiation. Studies on mammary gland in organ culture.

3. Extensive destruction of newly synthesized casein in mammary explants in organ culture.

4. Protein degradation in rat liver during post-natal development.

5. Casein turnover in rabbit mammary explants in organ culture.