The effect of prostaglandin E1 on hemopoiesis in long-term bone marrow cultures.

The effect of prostaglandin E1 (PGE1) on the proliferation and differentiation of hemopoietic stem cells has been studied in a long-term bone marrow liquid culture system. In cultures supplemented with PGE1, there was a dose dependent decrease in both CFUS and CFUc. (CSA levels rose dose dependently, with a concomitant increase in mature granulocyte production. Macrophage production was suppressed.) After cessation of PGE1 treatment, CFUS and CFUc returned gradually to the control values and CSA was not detectable. There was no significant effect on hemopoiesis in cultures supplemented with 10(-8) M or less concentrations.