Subpopulation of lymphocytes in the infiltrate of experimental sporotrichosis.

Lymphocyte subpopulations of dermal infiltrated cells on experimental sporotrichosis of guinea pigs was performed. According to Tan's method, infected sites were incubated with collagenase and then mechanically disaggregated. The liberated cells were used for determination of lymphocytes subpopulation. The predominance of B cells was present on the inoculated site, compare with blood from control and inoculated animals. Thus, the present result suggests the important role of humoral immunity in the development of sporotrichosis lesion.