Literature DB >> 69672

The use of immunoabsorbents for the purification of mycobacterial antigens.

T M Daniel, P A Anderson.   

Abstract

Affinity chromatography using immunoabsorbents prepared from goat antisera has been applied to the purificaton of antigens from culture filtrates of Mycobacterium tuberculosis. The optimum conditions for the preparation and use of these immunoabsorbents have been determined. Urea has been studied as an eluant, and it has been found that antigen can be eluted with 4M urea at pH 9 with satisfactory yields, minimal loss of antigenicity, and minimal disruption of the immunoabsorbent.

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Year:  1977        PMID： 69672

Source DB:  PubMed          Journal:  J Lab Clin Med        ISSN： 0022-2143


  13 in total

1.  Antigenic differences between extracts of actively replicating and synchronized resting cells of Mycobacterium tuberculosis.

Authors:  L G Wayne; H A Sramek
Journal:  Infect Immun       Date:  1979-05       Impact factor: 3.441

2.  Immunological method to differentiate between antigens of tubercle bacilli, other mycobacterial species, and non-acid-fast bacteria.

Authors:  H R Ferguson; J K McClatchy; T R Sharpton; P Minden
Journal:  Infect Immun       Date:  1978-10       Impact factor: 3.441

3.  Rapid diagnosis of tuberculous meningitis with a dot enzyme immunoassay to detect antibody in cerebrospinal fluid.

Authors:  A Mathai; V V Radhakrishnan; M Thomas
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1991-05       Impact factor: 3.267

4.  Demonstration of a shared epitope among mycobacterial antigens using a monoclonal antibody.

Authors:  T M Daniel; G R Olds
Journal:  Clin Exp Immunol       Date:  1985-05       Impact factor: 4.330

Review 5.  Mycobacterial antigens: a review of their isolation, chemistry, and immunological properties.

Authors:  T M Daniel; B W Janicki
Journal:  Microbiol Rev       Date:  1978-03

6.  Specificity of Mycobacterium tuberculosis antigen 5 determined with mouse monoclonal antibodies.

Authors:  T M Daniel; N J Gonchoroff; J A Katzmann; G R Olds
Journal:  Infect Immun       Date:  1984-07       Impact factor: 3.441

7.  A longitudinal study of antibody titres to Antigen 6 in patients with bone and joint tuberculosis.

Authors:  J H Lau; J C Leong; A B Stroebel
Journal:  Int Orthop       Date:  1983       Impact factor: 3.075

8.  Immunological evaluation of a component isolated from Mycobacterium bovis BCG with a monoclonal antibody to M. bovis BCG.

Authors:  P Minden; P J Kelleher; J H Freed; L D Nielsen; P J Brennan; L McPheron; J K McClatchy
Journal:  Infect Immun       Date:  1984-11       Impact factor: 3.441

9.  Suppression of lymphocyte responses by tuberculous plasma and mycobacterial arabinogalactan. Monocyte dependence and indomethacin reversibility.

Authors:  M E Kleinhenz; J J Ellner; P J Spagnuolo; T M Daniel
Journal:  J Clin Invest       Date:  1981-07       Impact factor: 14.808

10.  Isolation of two antigens from the culture filtrates of Mycobacterium tuberculosis and their applications in the laboratory diagnosis of the tuberculous meningitis.

Authors:  V V Krishnan; A Mathai
Journal:  Med Microbiol Immunol       Date:  1991       Impact factor: 3.402
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