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Literature DB >> 6964393

Instability of coated vesicles in concentrated sucrose solutions.

P K Nandi, G Irace, P P Van Jaarsveld, R E Lippoldt, H Edelhoch.

Abstract

A method of preparing homogeneous coated vesicles that eliminates the high sucrose concentrations heretofore used is presented. It is shown that sucrose at high concentrations dissociates the coat from coated vesicles. This reaction can explain the presence of empty coats observed with preparations obtained with high concentrations of sucrose. The protein and membrane lipid components have been analyzed by the intrinsic tryptophan and extrinsic diphenylhexatriene fluorescence, respectively. Analysis of mixtures of coated vesicles and baskets resolved the contributions of the two species to the fluorescence curves.

Entities: Chemical

Mesh：

Substances：

Year: 1982 PMID： 6964393 PMCID： PMC347014 DOI： 10.1073/pnas.79.19.5881

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

22 in total

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Journal: J Mol Biol Date: 1978-12-25 Impact factor: 5.469

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Journal: Cell Date: 1978-08 Impact factor: 41.582

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Authors: J Heuser
Journal: J Cell Biol Date: 1980-03 Impact factor: 10.539

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Authors: W W Franke; M R Lüder; J Kartenbeck; H Zerban; T W Keenan
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33 in total

1. Measuring the elasticity of clathrin-coated vesicles via atomic force microscopy.

Authors: Albert J Jin; Kondury Prasad; Paul D Smith; Eileen M Lafer; Ralph Nossal
Journal: Biophys J Date: 2006-02-10 Impact factor: 4.033

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3. In vitro insertion of the myelin proteolipid apoprotein into oligodendrocyte plasma membranes.

Authors: Z Chraibi; A Alfsen; F Lavialle
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Authors: A Passaniti; T F Roth
Journal: Biochem J Date: 1989-03-01 Impact factor: 3.857

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Authors: L R Pierce; C Zurzolo; G Salvatore; H Edelhoch
Journal: J Endocrinol Invest Date: 1985-08 Impact factor: 4.256

6. Brain clathrin light chain 2 can be phosphorylated by a coated vesicle kinase.

Authors: W J Schook; S Puszkin
Journal: Proc Natl Acad Sci U S A Date: 1985-12 Impact factor: 11.205

7. Uncoating of clathrin-coated vesicles by uncoating ATPase from developing peas.

Authors: T Kirsch; L Beevers
Journal: Plant Physiol Date: 1993-09 Impact factor: 8.340

8. Differential functions of ApoER2 and very low density lipoprotein receptor in Reelin signaling depend on differential sorting of the receptors.

Authors: Sarah Duit; Harald Mayer; Sophia M Blake; Wolfgang J Schneider; Johannes Nimpf
Journal: J Biol Chem Date: 2009-11-30 Impact factor: 5.157

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Authors: T Saermark; P M Jones; I C Robinson
Journal: Biochem J Date: 1984-03-01 Impact factor: 3.857

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Authors: R W Van Dyke; C J Steer; B F Scharschmidt
Journal: Proc Natl Acad Sci U S A Date: 1984-05 Impact factor: 11.205