Literature DB >> 6957705

A sensitive radioimmuno assay for thymine dimers.

H Klocker, B Auer, H J Burtscher, J Hofmann, M Hirsch-Kauffmann, M Schweiger.   

Abstract

A sensitive radioimmuno assay (RIA) method for detection of the UV photoproduct, thymine dimers (TT) has been developed. The limit of detection of this method is 6 X 10(-14) mol or 15 pg thymine dimer. It is highly specific: A structurally similar compound such as uridine dimer (UU) interferes with the detection of thymine dimers only when it is 53,000-fold or more in molar excess. Since this RIA method does not require the use of labeled DNA, it represents a considerable improvement for repair studies with radiation-sensitive cells.

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Year:  1982        PMID： 6957705     DOI： 10.1007/bf00337950

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  17 in total

1.  Isolation and identification of the irradiation product of thymine.

Authors:  R BEUKERS; W BERENDS
Journal:  Biochim Biophys Acta       Date:  1960-07-15

2.  DNA repair: new clues to carcinogenesis.

Authors:  J L Marx
Journal:  Science       Date:  1978-05-05       Impact factor: 47.728

3.  Micrococcus luteus correndonucleases. I. resolution and purification of two endonucleases specific for DNA containing pyrimidine dimers.

Authors:  S Riazuddin; L Grossman
Journal:  J Biol Chem       Date:  1977-09-25       Impact factor: 5.157

Review 4.  Repair deficient human disorders and cancer.

Authors:  R B Setlow
Journal:  Nature       Date:  1978-02-23       Impact factor: 49.962

5.  Methods for the assay of ultraviolet light-induced pyrimidine dimers in Saccharomyces cerevisiae.

Authors:  P Unrau; R Wheatcroft; B S Cox
Journal:  Biochim Biophys Acta       Date:  1972-05-29

6.  Immunological demonstration of the disappearance of pyrimidine dimers from nuclei of cultured human cells.

Authors:  C J Lucas
Journal:  Exp Cell Res       Date:  1972-10       Impact factor: 3.905

7.  Preferential DNA repair in human cells.

Authors:  R J Wilkins; R W Hart
Journal:  Nature       Date:  1974-01-04       Impact factor: 49.962

8.  Serologic estimation of thymine dimers in the deoxyribonucleic acid of bacterial and mammalian cells following irradiation with ultraviolet light and postirradiation repair.

Authors:  E Seaman; H Van Vunakis; L Levine
Journal:  J Biol Chem       Date:  1972-09-25       Impact factor: 5.157

9.  The development of a radioimmunoassay for the detection of photoproducts in mammalian cell DNA.

Authors:  D L Mitchell; J M Clarkson
Journal:  Biochim Biophys Acta       Date:  1981-08-27

10.  Fluorescent-light-induced lethality and DNA repair in normal and xeroderma pigmentosum fibroblasts.

Authors:  M A Ritter; J R Williams
Journal:  Biochim Biophys Acta       Date:  1981-08-27
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  6 in total

1.  Excision of pyrimidine dimers from the DNA of Neurospora.

Authors:  H Macleod; D Stadler
Journal:  Mol Gen Genet       Date:  1986-02

2.  Repair rate in human fibroblasts measured by thymine dimer excorporation.

Authors:  H Klocker; B Auer; H J Burtscher; M Hirsch-Kauffmann; M Schweiger
Journal:  Mol Gen Genet       Date:  1982

3.  Poly-ADP-ribosylation is not required for excision of a DNA damage.

Authors:  H Klocker; H J Burtscher; B Auer; M Hirsch-Kauffmann; M Schweiger
Journal:  Naturwissenschaften       Date:  1983-02

4.  Clinical and biochemical studies in three patients with severe early infantile Cockayne syndrome.

Authors:  J Jaeken; H Klocker; H Schwaiger; R Bellmann; M Hirsch-Kauffmann; M Schweiger
Journal:  Hum Genet       Date:  1989-11       Impact factor: 4.132

5.  Ultraviolet Light Inhibition of Phytochrome-Induced Flavonoid Biosynthesis and DNA Photolyase Formation in Mustard Cotyledons (Sinapis alba L.).

Authors:  G. Buchholz; B. Ehmann; E. Wellmann
Journal:  Plant Physiol       Date:  1995-05       Impact factor: 8.340

6.  UV-induced photoproducts of 5-methylcytosine in a DNA sequence context.

Authors:  T Barna; J Malinowski; P Holton; M Ruchirawat; F F Becker; J N Lapeyre
Journal:  Nucleic Acids Res       Date:  1988-04-25       Impact factor: 16.971
  6 in total

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