Literature DB >> 6956336

Assay of free and glycine- and taurine-conjugated bile acids in serum by high-pressure liquid chromatography by using post-column reaction after group separation.

S Onishi, S Itoh, Y Ishida.   

Abstract

An accurate and sensitive method that involves the group separations of serum bile acids (i.e. free and glycine- and taurine-conjugated bile acid fractions) by ion-exchange chromatography on piperidinohydroxypropyl-Sephadex LH-20 is described. Each group was then analysed by high-pressure liquid chromatography by using the post-column reaction technique with immobilized 3 alpha-hydroxy steroid dehydrogenase. The bile acid patterns in the umbilical venous serum samples were analysed by this method. Taurochenodeoxycholate predominated in the umbilical blood.

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Year:  1982        PMID: 6956336      PMCID: PMC1158324          DOI: 10.1042/bj2040135

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  6 in total

1.  Covalent coupling methods for inorganic support materials.

Authors:  H H Weetall
Journal:  Methods Enzymol       Date:  1976       Impact factor: 1.600

2.  Analysis of metabolic profiles of bile acids in urine using a lipophilic anion exchanger and computerized gas-liquid chromatorgaphy-mass spectrometry.

Authors:  B Almé; A Bremmelgaard; J Sjövall; P Thomassen
Journal:  J Lipid Res       Date:  1977-05       Impact factor: 5.922

3.  Bile acids. XLVIII. Separation of conjugated bile acids by high-pressure liquid chromatography.

Authors:  R Shaw; W H Elliott
Journal:  Anal Biochem       Date:  1976-08       Impact factor: 3.365

4.  Serum bile acids in newborns and children.

Authors:  L Barbara; R Lazzari; A Roda; R Aldini; D Festi; C Sama; A M Morselli; A Collina; F Bazzoli; G Mazzella; E Roda
Journal:  Pediatr Res       Date:  1980-11       Impact factor: 3.756

5.  Fetomaternal relationships of serum bile acids in uncomplicated pregnancy.

Authors:  T Laatikainen
Journal:  Scand J Clin Lab Invest       Date:  1977-11       Impact factor: 1.713

6.  A new method for simultaneous determination of bile acids in human bile without hydrolysis.

Authors:  J Goto; M Hasegawa; H Kato; T Nambara
Journal:  Clin Chim Acta       Date:  1978-07-01       Impact factor: 3.786

  6 in total
  4 in total

Review 1.  Isolation and determination of bile acids.

Authors:  J Kandrac; S Kevresan; J K Gu; M Mikov; J P Fawcett; K Kuhajda
Journal:  Eur J Drug Metab Pharmacokinet       Date:  2006 Jul-Sep       Impact factor: 2.441

Review 2.  A review of analytical platforms for accurate bile acid measurement.

Authors:  Mainak Dutta; Jingwei Cai; Wei Gui; Andrew D Patterson
Journal:  Anal Bioanal Chem       Date:  2019-05-24       Impact factor: 4.142

3.  Foetomaternal relationships of serum bile acid pattern estimated by high-pressure liquid chromatography.

Authors:  S Itoh; S Onishi; K Isobe; M Manabe; K Inukai
Journal:  Biochem J       Date:  1982-04-15       Impact factor: 3.857

Review 4.  Bile Acid Detection Techniques and Bile Acid-Related Diseases.

Authors:  Xiang Zhao; Zitian Liu; Fuyun Sun; Lunjin Yao; Guangwei Yang; Kexin Wang
Journal:  Front Physiol       Date:  2022-03-16       Impact factor: 4.566

  4 in total

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