Differences in lymphoproliferative responses to the bacterium Actinomyces viscosus in various mammalian species.

A water-soluble extract of Actinomyces viscosus (AVS) was tested for its capacity to induce DNA synthesis in lymphocytes from man, monkeys, mice and guinea pigs. The results indicated that the AVS induced an in-vitro lymphoproliferative response, as assessed by tritiated thymidine incorporation, in mouse-spleen cells, in the majority of human peripheral blood samples tested and in macaque monkey spleen cells. The AVS also elicited a blastogenic response from spleen, lymph node and peripheral blood lymphocytes from guinea pigs immunized with A. viscosus. The AVS did not elicit a lymphoproliferative response from human-cord blood cells, monkey peripheral blood lymphocytes, or peripheral blood and spleen lymphocytes from non-immunized guinea pigs. Thus there was a difference in the ability of A. viscosus to induce DNA synthesis in lymphocytes from the different animal species tested.