Literature DB >> 6948295

Energy-filtered transmission electron microscopy of ferritin.

H Shuman, A P Somlyo.   

Abstract

The focusing properties of a magnetic-sector spectrometer are shown to be suitable for forming high-spatial-resolution, energy-filtered transmission electron microscope images. Filtered images of ferritin molecules by using electrons scattered from the characteristic iron M2,3 and carbon K absorption edges clearly distinguish the 75-A iron core and 120-A protein shell. The minimum detectable mass is estimated to be 0.84 X 10(-20) g for Fe for an electron dose of 18 C/cm2 and 99% confidence.

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Year:  1982        PMID: 6948295      PMCID: PMC345670          DOI: 10.1073/pnas.79.1.106

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN: 0027-8424            Impact factor:   11.205


  11 in total

1.  Electron microspectroscopy.

Authors:  M S Isaacson; A V Crewe
Journal:  Annu Rev Biophys Bioeng       Date:  1975

2.  Electron probe x-ray analysis of single ferritin molecules.

Authors:  H Shuman; A P Somlyo
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

3.  The microanalysis of light elements using transmitted energy loss electrons.

Authors:  M Isaacson
Journal:  Ultramicroscopy       Date:  1975-07       Impact factor: 2.689

4.  Electron probe analysis of muscle and X-ray mapping of biological specimens with a field emission gun.

Authors:  A P Somlyo; A V Somlyo; H Shuman; M Stewart
Journal:  Scan Electron Microsc       Date:  1979

5.  The ultrastructure of ferritin macromolecules. III. Mineralized iron in ferritin is attached to the protein shell.

Authors:  W H Massover
Journal:  J Mol Biol       Date:  1978-08-25       Impact factor: 5.469

6.  Formulae for light-element microanalysis by electron energy-loss spectrometry.

Authors:  R F Egerton
Journal:  Ultramicroscopy       Date:  1978       Impact factor: 2.689

7.  The use of electron energy loss spectroscopy for studying membrane architecture: a preliminary report.

Authors:  J Hainfeld; M Isaacson
Journal:  Ultramicroscopy       Date:  1978       Impact factor: 2.689

8.  Distribution of calcium and other elements in cryosectioned Bacillus cereus T spores, determined by high-resolution scanning electron probe x-ray microanalysis.

Authors:  M Stewart; A P Somlyo; A V Somlyo; H Shuman; J A Lindsay; W G Murrell
Journal:  J Bacteriol       Date:  1980-07       Impact factor: 3.490

9.  Parallel recording of electron energy loss spectra.

Authors:  H Shuman
Journal:  Ultramicroscopy       Date:  1981       Impact factor: 2.689

10.  Correction of the second-order aberrations of uniform field magnetic sectors.

Authors:  H Shuman
Journal:  Ultramicroscopy       Date:  1980       Impact factor: 2.689

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  2 in total

1.  Ultrastructure, function and composition of smooth muscle.

Authors:  A P Somlyo; A V Somlyo; T Kitazawa; M Bond; H Shuman; D Kowarski
Journal:  Ann Biomed Eng       Date:  1983       Impact factor: 3.934

2.  3D morphology of the human hepatic ferritin mineral core: new evidence for a subunit structure revealed by single particle analysis of HAADF-STEM images.

Authors:  Ying-Hsi Pan; Kasim Sader; Jonathan J Powell; Andrew Bleloch; Mhairi Gass; John Trinick; Alice Warley; Andy Li; Rik Brydson; Andy Brown
Journal:  J Struct Biol       Date:  2008-12-13       Impact factor: 2.867

  2 in total

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