Literature DB >> 694728

Effect of intercellular communication on the selection of intraspecific human hybrids in HAT and ouabain.

C M Corsaro, B R Migeon.   

Abstract

Optimal conditions have been determined for selection of hybrids in HAT and ouabain (HOT) from the PEG-mediated fusion of OUAR/HGPRT-/G6PDA and OUAS/HGPRT+/G6PDB human diploid fibroblasts. In addition to the OUARHGPRT+G6PDAB tetraploid hybrids, several other types of HOT-resistant colonies were obtained from these fusions and identified by G6PD isozyme analysis and karyotyping as (1) hybrids contaminated with diploid parental cells, (2) symbiotic mixtures of the two types of parental cells, or (3) spontaneous OUAR mutants. The mechanism for parental cell growth in HOT is most likely the transfer of HAT resistance and/or ouabain resistance through gap junctions formed between resistant and sensitive cells.

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Year:  1978        PMID： 694728     DOI： 10.1007/BF01542925

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  5 in total

1.  DNA restriction endonuclease analysis for localization of human beta- and delta-globin genes on chromosome 11.

Authors:  A F Scott; J A Phillips; B R Migeon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  Complementation of multiple sulfatase deficiency in somatic cell hybrids.

Authors:  K Fedde; A L Horwitz
Journal:  Am J Hum Genet       Date:  1984-05       Impact factor: 11.025

3.  Studies of the locus for androgen receptor: localization on the human X chromosome and evidence for homology with the Tfm locus in the mouse.

Authors:  B R Migeon; T R Brown; J Axelman; C J Migeon
Journal:  Proc Natl Acad Sci U S A       Date:  1981-10       Impact factor: 11.205

4.  Localization of loci for hypoxanthine phosphoribosyltransferase and glucose-6-phosphate dehydrogenase and biochemical evidence of nonrandom X chromosome expression from studies of a human X-autosome translocation.

Authors:  G S Pai; J A Sprenkle; T T Do; C E Mareni; B R Migeon
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

5.  Coordinate expression of parietal endodermal functions in hybrids of embryonal carcinoma and endodermal cells.

Authors:  W E Howe; R G Oshima
Journal:  Mol Cell Biol       Date:  1982-03       Impact factor: 4.272
  5 in total

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