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Literature DB >> 6946448

Periodic synthesis of microtubular proteins in the cell cycle of Physarum.

Abstract

Periodic polypeptide labeling over the naturally synchronous nuclear replication cycle of Physarum polycephalum was analyzed by fluorography of two-dimensional electropherograms. Two sets of polypeptides, denoted as P and Q, showed strong periodicity; they were maximally labeled just prior to mitosis. This periodicity was shown to reflect synthesis rather than turnover or recovery. Both P and Q copolymerized with porcine microtubular proteins and displayed electrophoretic properties similar to those of porcine tubulins. The significance of the periodic synthesis of these microtubular proteins is discussed as a possible component within the chain of events that establishes the high mitotic synchrony of Physarum syncytia.

Entities: Disease Species

Mesh：

Substances：
Tubulin

Year: 1981 PMID： 6946448 PMCID： PMC320319 DOI： 10.1073/pnas.78.8.5000

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

19 in total

8. An immunological approach to enrich a mitotic stimulator and to reveal G2-phase-specific proteins in Physarum polycephalum.

Authors: P Gröbner; P Loidl
Journal: J Cell Biol Date: 1985-06 Impact factor: 10.539

9. Transcription of alpha-tubulin and histone H4 genes begins at the same point in the Physarum cell cycle.

Authors: J J Carrino; T G Laffler
Journal: J Cell Biol Date: 1986-05 Impact factor: 10.539

10. Cell type-dependent expression of tubulins in Physarum.

Authors: T G Burland; K Gull; T Schedl; R S Boston; W F Dove
Journal: J Cell Biol Date: 1983-12 Impact factor: 10.539

10 in total

Periodic synthesis of microtubular proteins in the cell cycle of Physarum.

1. Visualization of proteins after isoelectric focusing during two-dimensional gel electrophoresis.

2. Mechanisms of regulating tubulin synthesis in cultured mammalian cells.

3. Induction and synthesis of tubulin during the cell cycle and life cycle of Chlamydomonas reinhardi.

4. Changes in the synthesis of actin and other cell proteins after stimulation of serum-arrested cells.

5. In vitro assembly of microtubule proteins from myxamoebae of Physarum polycephalum.

6. Characterization and in vitro polymerization of Tetrahymena tubulin.

7. Purification and properties of the sigma subunit of Escherichia coli DNA-dependent RNA polymerase.

8. Rate of macromolecular synthesis through the cell cycle of the yeast Saccharomyces cerevisiae.

9. ORGANIC REQUIREMENTS AND SYNTHETIC MEDIA FOR GROWTH OF THE MYXOMYCETE PHYSARUM POLYCEPHALUM.

10. A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells.

1. Cell cycle stage-specific accumulation of mRNAs encoding tubulin and other polypeptides in Chlamydomonas.

2. A cell-cycle-dependent protein of Physarum polycephalum revealed by two-dimensional gel electrophoresis.

3. Photomorphogenesis in Physarum: induction of tubulins and sporulation-specific proteins and of their mRNAs.

4. Distinct replication-independent and -dependent phases of histone gene expression during the Physarum cell cycle.

5. Cell-cycle-regulated translation of histone mRNA in Physarum plasmodia.

6. Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum.

7. The effect of heat shock on the cell cycle regulation of tubulin expression in Physarum polycephalum.

8. An immunological approach to enrich a mitotic stimulator and to reveal G2-phase-specific proteins in Physarum polycephalum.

9. Transcription of alpha-tubulin and histone H4 genes begins at the same point in the Physarum cell cycle.

10. Cell type-dependent expression of tubulins in Physarum.