Differences in types of prostaglandins produced by two MOCK canine kidney cell sublines.

The pattern of prostaglandins produced from arachidonic acid by two sublines of MDCK canine kidney epithelial cells was different. In one subline designated MDCK1, the most prevalent prostaglandin product was PGE2, whereas the most prevalent product in the subline designated MDCK2 was PGF2 alpha. This difference was observed when cells previously labeled with [1-14C]arachidonic acid were stimulated with either bradykinin or the calcium ionophore A23187, or when prostaglandins were produced from labeled arachidonic acid added directly to the assay medium. In the latter case, the difference was maintained over a 38-fold range of extracellular arachidonate concentrations. These findings indicate that there is a persistent difference in the distribution of prostaglandins produced by the two commonly used sublines of MDCK cells.