Literature DB >> 6941897

On the production of 12 alpha-hydroxysteroid dehydrogenase from Clostridium group P, strain C48-50 ATCC 29733.

I A Macdonald.   

Abstract

The production of 12 alpha-hydroxysteroid dehydrogenase of Clostridium group P strain C48-50 was optimized when the organism was grown in the presence of 2% fructose and 0.1% dithiothreitol. It appears that an initial redox potential of less than -160 mV (achieved by autoclaving in the presence of dithiothreitol, dithioerythritol or cysteine) is important in the production of this enzyme.

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Year:  1981        PMID： 6941897     DOI： 10.1007/bf01986126

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  6 in total

1.  Partial purification and characterization of NADP-dependent 12alpha-hydroxysteroid dehydrogenase from Clostridium leptum.

Authors:  J N Harris; P B Hylemon
Journal:  Biochim Biophys Acta       Date:  1978-01-27

2.  Bile salt degradation by nonfermentative clostridia.

Authors:  D E Mahony; C E Meier; I A Macdonald; L V Holdeman
Journal:  Appl Environ Microbiol       Date:  1977-10       Impact factor: 4.792

3.  New markers for Eubacterium lentum.

Authors:  V D Bokkenheuser; J Winter; S M Finegold; V L Sutter; A E Ritchie; W E Moore; L V Holdeman
Journal:  Appl Environ Microbiol       Date:  1979-05       Impact factor: 4.792

4.  12alpha-Hydroxysteroid dehydrogenase from Clostridium group P strain C48-50 ATCC No. 29733: partial purification and characterization.

Authors:  I A Macdonald; J F Jellett; D E Mahony
Journal:  J Lipid Res       Date:  1979-02       Impact factor: 5.922

5.  Fecal hydroxysteroid dehydrogenase activities in vegetarian Seventh-Day Adventists, control subjects, and bowel cancer patients.

Authors:  I A Macdonald; G R Webb; D E Mahony
Journal:  Am J Clin Nutr       Date:  1978-10       Impact factor: 7.045

6.  Enzymic determination of 3 alpha-, 7 alpha-, and 12 alpha-hydroxyl groups of fecal bile salts.

Authors:  M J Crowell; I A Macdonald
Journal:  Clin Chem       Date:  1980-08       Impact factor: 8.327
  6 in total

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